Siglec-1 Fc Chimera Active Protein

Siglec-1 Fc Chimera, Recombinant, Mouse (Sialic acid binding Ig-like lectin 1, Sialoadhesin, CD169, dJ1009E24.1, DKFZp667F058, FLJ00051, FLJ00055, FLJ00073, FLJ00411, FLJ32150, Siglec-1, SN)

Cat. Num. AAA636430

• Reactivity: Mouse
• Applications: ELISA
• Purity: Highly Purified; 90% by SDS­PAGE under reducing conditions and visualized by silver stain.
• Synonyms: Siglec-1 Fc Chimera; Recombinant; Mouse (Sialic acid binding Ig-like lectin 1; Sialoadhesin; CD169; dJ1009E24.1; DKFZp667F058; FLJ00051; FLJ00055; FLJ00073; FLJ00411; FLJ32150; Siglec-1; SN); Siglec-1 Fc Chimera active protein

• Reactivity: Mouse
• Specificity: Recognizes mouse Siglec-1.
• Purity/Purification: Highly Purified; 90% by SDS­PAGE under reducing conditions and visualized by silver stain.
• Form/Format: Lyophilized from a 0.2um filtered solution in PBS. Reconstitute at 100ug/ml in PBS.

• Applicable Applications for Siglec-1 Fc Chimera active protein: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA.
• Activity: Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. The ED50 for this effect is typically ~0.6ug/ml.
• Endotoxin Level: 1.0 EU per 1ug of the protein by the LAL method.
• Preparation and Storage: 12 months from date of receipt, 20 to 70 degree C as supplied.; 1 month, 2 to 8 degree C under sterile conditions after reconstitution.; 3 months, 20 to 70 degree C under sterile conditions after reconstitution.; Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Related Product Information for Siglec-1 Fc Chimera active protein

Siglecs are sialic acid specific I­t ype lectins that belong to the immunoglobulin superfamily. Structurally, they are transmembrane proteins with an N­terminal Ig ­like V­set domain followed by varying numbers of Ig­like C2­set domains (1, 2). Mouse Siglec­1, also known as sialoadhesin and CD169, is a 175 ­185kD glycoprotein that consists of a 1619 amino acid (aa) extracellular domain (ECD) with one Ig­like V­set domain and 16 Ig­like C2­set domains, a 21 aa transmembrane segment, and a 35 aa cytoplasmic domain (3, 4). Within the ECD, mouse Siglec­1 shares 73% and 83% aa sequence identity with human and rat Siglec­1, respectively. Alternate splicing generates a soluble form of the ECD and a soluble isoform that is truncated following the first three Ig­like domains (3). Siglec­1 expression is restricted to lymphnode and spleen macrophages and some tissue macrophages (4). The adhesive function of Siglec­1 is supported by the N­terminal Ig ­like domain which shows a selectivity for ­2, 3­linked sialic acid residues (4­6). Siglec­1 binds a number of sialylated molecules including the mannose receptor, MGL1, MUC1, PSGL­1, and different glycoforms of CD43 (7­10). Its binding capacity can be masked by endogenous sialylated molecules (11, 12). The sialylated and sulfated N­linked carbohydrates that modify Siglec­1 itself are required for ligand binding (7,8). Siglec­1 is expressed on dendritic cells following rhinovirus exposure, and these DC promote T cell anergy (13). It is also induced on circulating monocytes during systemic sclerosis and HI V­1 infection (14­16). Siglec­1 can trap HI V­1 particles for trans infection of permissive cells (15).

Product Categories/Family for Siglec-1 Fc Chimera active protein

Molecular Biology; MB-Lectins