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Western Blot (WB) (Western blot detection of Wnt2b in K562,C6,3T3,Hela cell lysates using Wnt2b Rabbit mAb(1:1000 diluted). Predicted band size:44kDa. Observed band size:44kDa.)

Rabbit Wnt2b Monoclonal Antibody | anti-WNT2B antibody

Wnt2b Rabbit mAb

Gene Names
WNT2B; WNT13
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Affinity purified
Synonyms
Wnt2b; Monoclonal Antibody; Wnt2b Rabbit mAb; Protein Wnt-2b; anti-WNT2B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
R01-8F5
Specificity
Isoform 1 is expressed in adult heart, brain, placenta, lung, prostate, testis, ovary, small intestine and colon. In the adult brain, it is mainly found in the caudate nucleus, subthalamic nucleus and thalamus. Also detected in fetal brain, lung and kidney. Isoform 2 is expressed in fetal brain, fetal lung, fetal kidney, caudate nucleus, testis and cancer cell lines. Gene expression databases
Purity/Purification
Affinity purified
Form/Format
Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-WNT2B antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
ICC/IF: 1:20
FC/FACS: 1:20
Immunogen
A synthetic peptide of human Wnt2b
Conjugation
Unconjugated
Modification
Unmodification
Subcellular Location
Extracellular matrix, Secreted
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot detection of Wnt2b in K562,C6,3T3,Hela cell lysates using Wnt2b Rabbit mAb(1:1000 diluted). Predicted band size:44kDa. Observed band size:44kDa.)

Western Blot (WB) (Western blot detection of Wnt2b in K562,C6,3T3,Hela cell lysates using Wnt2b Rabbit mAb(1:1000 diluted). Predicted band size:44kDa. Observed band size:44kDa.)
Related Product Information for anti-WNT2B antibody
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
41,830 Da
NCBI Official Full Name
Protein Wnt-2b
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 2B
NCBI Official Symbol
WNT2B
NCBI Official Synonym Symbols
WNT13
NCBI Protein Information
protein Wnt-2b; XWNT2, Xenopus, homolog of; wingless-type MMTV integration site family, member 13
UniProt Protein Name
Protein Wnt-2b
Protein Family
UniProt Gene Name
WNT2B
UniProt Synonym Gene Names
WNT13
UniProt Entry Name
WNT2B_HUMAN

NCBI Description

This gene encodes a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. This gene may play a role in human development as well as carcinogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

WNT2B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: frizzled binding

Biological Process: neuron differentiation; lens development in camera-type eye; Wnt receptor signaling pathway; cell fate commitment; male gonad development; chondrocyte differentiation; Wnt receptor signaling pathway through beta-catenin; forebrain regionalization; cellular response to starvation

Research Articles on WNT2B

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Product Notes

The WNT2B wnt2b (Catalog #AAA4753103) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Wnt2b Rabbit mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Wnt2b can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 1:1000 ICC/IF: 1:20 FC/FACS: 1:20. Researchers should empirically determine the suitability of the WNT2B wnt2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Wnt2b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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