Human Tumor Necrosis Factor alpha IgG (TNFA-Ab-IgG) ELISA Kit | TNFA-Ab-IgG elisa kit

Human Tumor Necrosis Factor alpha IgG Antibody (TNFA-Ab-IgG) ELISA Kit

Cat. Num. AAA9716188

• Reactivity: Human
• Synonyms: Tumor Necrosis Factor alpha IgG (TNFA-Ab-IgG); Human Tumor Necrosis Factor alpha IgG Antibody (TNFA-Ab-IgG) ELISA Kit; TNFA-Ab-IgG; antibody for DADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein; TNF superfamily; member 2; TNF; macrophage-derived; monocyte-derived; cachectin; tumor necrosis factor alpha; TNFA-Ab-IgG elisa kit

• Reactivity: Human
• Specificity: Human Tumor necrosis factor alpha IgG antibody (TNFA-Ab-IgG) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNFA-Ab-IgG. No significant cross-reactivity or interference between Human TNFA-Ab-IgG and analogues was observed.

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Tumor necrosis factor alpha IgG antibody (TNFA-Ab-IgG) Standard Curve.)

Related Product Information for TNFA-Ab-IgG elisa kit

Description: This Human Tumor necrosis factor alpha IgG antibody (TNFA-Ab-IgG) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFA-Ab-IgG in samples. An antibody specific for TNFA-Ab-IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFA-Ab-IgG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFA-Ab-IgG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFA-Ab-IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: TNFa is synthesized as a 26 kDa, type II transmembrane protein that is 233 amino acids in length. It contains a 30 amino acid (aa) cytoplasmic domain, a 26 aa transmembrane segment, and a 177 aa extracellular region. TNFa is assembled intracellularly to form a transmembrane, non-covalently-linked homotrimeric protein. The 157 aa residue soluble form of TNFa (sTNF-alphais released from the C-terminus of the transmembrane protein through the activity of TNFa-converting enzyme (TACE), a membrane -bound disintegrin metalloproteinase. Rat cells known to express TNF-alphainclude B cells, colonic columnar epithelial cells, NK and CD3 CD56 hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4 and CD8 T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes.