Mouse anti-Human Wnt Inhibiting Factor 1 Monoclonal Antibody | anti-Wisp1 antibody

Wnt Inhibiting Factor 1 (WIF-1)

Cat. Num. AAA601770

• Gene Names: Wisp1; CCN4; Elm1; AW146261
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Wnt Inhibiting Factor 1; Monoclonal Antibody; Wnt Inhibiting Factor 1 (WIF-1); Anti -Wnt Inhibiting Factor 1 (WIF-1); anti-Wisp1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8H102
• Specificity: Recognizes human Wnt Inhibiting Factor 1. Shows less than 5% crossreactivity with recombinant mouse WIF-1.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 100ul sterile PBS.

• Applicable Applications for anti-Wisp1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1ug/ml; Immunohistochemistry (Paraffin): 8-25ug/ml; Used to detect WIF-1 in immersion fixed paraffin-embedded human breast sections.
• Immunogen: Recombinant protein corresponding to aa29-379 from human WIF-1, expressed in NSO cells. (Accession #AAD25402).
• Hybridoma: Mouse myeloma with B cells from a mouse immunized with purified, NS0-derived, recombinant human WIF-1.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(WIF‑1 in Human Breast. WIF‑1 was detected in immersion fixed paraffin-embedded sections of human breast using 8ug/mL MBS601770)

Related Product Information for anti-Wisp1 antibody

WIF-1 is a secreted protein that binds Wnt proteins and antagonizes Wnt-induced signaling. WIF-1 expression is down regulated in several human cancers.

Product Categories/Family for anti-Wisp1 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 9256533
• NCBI GeneID: 22402
• NCBI Accession #: NP_061353.1
• NCBI GenBank Nucleotide #: NM_018865.2
• UniProt Accession #: O54775; Q80ZL1
• Molecular Weight: 40,703 Da
• NCBI Official Full Name: WNT1-inducible-signaling pathway protein 1
• NCBI Official Synonym Full Names: WNT1 inducible signaling pathway protein 1
• NCBI Official Symbol: Wisp1
• NCBI Official Synonym Symbols: CCN4; Elm1; AW146261
• NCBI Protein Information: WNT1-inducible-signaling pathway protein 1; ELM-1; WISP-1; CCN family member 4
• UniProt Protein Name: WNT1-inducible-signaling pathway protein 1
• Protein Family: WNT1-inducible-signaling pathway protein
• UniProt Gene Name: Wisp1
• UniProt Synonym Gene Names: Ccn4; Elm1; WISP-1
• UniProt Entry Name: WISP1_MOUSE

Uniprot Description

WISP1: Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. Belongs to the CCN family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Oncoprotein; Secreted

Cellular Component: proteinaceous extracellular matrix; cytoplasm; extracellular region

Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding

Biological Process: Wnt receptor signaling pathway; cell-cell signaling; regulation of cell growth; cell adhesion; signal transduction