WNT1 Inducible Signaling Pathway Protein 1 (WISP1) Recombinant Protein | WISP1 recombinant protein

Recombinant WNT1 Inducible Signaling Pathway Protein 1 (WISP1)

Cat. Num. AAA2010134

• Gene Names: Wisp1; CCN4; Elm1; AW146261
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: WNT1 Inducible Signaling Pathway Protein 1 (WISP1); Recombinant WNT1 Inducible Signaling Pathway Protein 1 (WISP1); WISP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-YENCIAYTSP WSPCSTTCGL GISTRISNVN ARCWPEQESR LCNLRPCDVD IQLHIKAGKK CLAVYQPEEA TNFTLAGCVS TRTYRPKYCG VCTDNRCCIP YKSKTISVDF QCPEGPGFSR QVLWINACFC NLSCRNPN
• Sequence Length: 367

• Applicable Applications for WISP1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Tyr213~Asn350 (Accession # O54775) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 9256533
• NCBI GeneID: 22402
• NCBI Accession #: NP_061353.1
• NCBI GenBank Nucleotide #: NM_018865.2
• UniProt Accession #: O54775; Q80ZL1
• Molecular Weight: 17.0kDa
• NCBI Official Full Name: WNT1-inducible-signaling pathway protein 1
• NCBI Official Synonym Full Names: WNT1 inducible signaling pathway protein 1
• NCBI Official Symbol: Wisp1
• NCBI Official Synonym Symbols: CCN4; Elm1; AW146261
• NCBI Protein Information: WNT1-inducible-signaling pathway protein 1; ELM-1; WISP-1; CCN family member 4
• UniProt Protein Name: WNT1-inducible-signaling pathway protein 1
• Protein Family: WNT1-inducible-signaling pathway protein
• UniProt Gene Name: Wisp1
• UniProt Synonym Gene Names: Ccn4; Elm1; WISP-1
• UniProt Entry Name: WISP1_MOUSE

Uniprot Description

WISP1: Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. Belongs to the CCN family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Oncoprotein

Cellular Component: proteinaceous extracellular matrix; cytoplasm; extracellular region

Molecular Function: integrin binding; heparin binding; insulin-like growth factor binding

Biological Process: Wnt receptor signaling pathway; cell-cell signaling; regulation of cell growth; cell adhesion; signal transduction