Mouse anti-Human, Mouse Viperin Monoclonal Antibody | anti-RSAD2 antibody

Viperin (Radical S-adenosyl Methionine Domain-containing Protein 2, Virus Inhibitory Protein, Endoplasmic Reticulum-associated, Interferon-inducible, Rsad2, Vig1)

Cat. Num. AAA6001804

• Gene Names: RSAD2; cig5; vig1; cig33; 2510004L01Rik
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Viperin; Monoclonal Antibody; Viperin (Radical S-adenosyl Methionine Domain-containing Protein 2; Virus Inhibitory Protein; Endoplasmic Reticulum-associated; Interferon-inducible; Rsad2; Vig1); Anti -Viperin (Radical S-adenosyl Methionine Domain-containing Protein 2; anti-RSAD2 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 13A88
• Specificity: Recognizes mouse Viperin. Species Crossreactivity: human.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

• Applicable Applications for anti-RSAD2 antibody: Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Flow Cytometry, Immunofluorescence and Immunoprecipitation. Not suitable for use in Functional Studies.; Dilution: Flow Cytometry: 1:50; Western Blot: 1:50
• Immunogen: Recombinant corresponding to aa92-362 from mouse viperin.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-RSAD2 antibody

Mouse Viperin is a 42kD protein belonging to the RSAD2 family. Viperin is the abbreviation of Virus inhibitory protein endoplasmic reticulum-associated interferon-inducible. It is an evolutionary conserved protein that is highly inducible by both type I and type II interferons. However, little or no induction by interferon gamma is observed in monocytic cell lines. Infection by many viruses, including human cytomegalovirus (hCMV), hepatitis C virus, yellow fever virus and Sendai virus, strongly induces viperin expression, suggesting a role in the host antiviral response. Viperin has also been shown to be important for the host anti-HIV responses. Furthermore, expression of viperin inhibits influenza replication by perturbing its release from the plasma membrane. Viperin expression alters plasma membrane fluidity by affecting formation of lipid rafts which are detergent-resistant membrane microdomains known to be the sites of influenza virus budding. HCMV infection induces the redistribution of viperin from the endoplasmic reticulum to the Golgi complex and ultimately to cytoplasmic vacuoles, suggesting that viperin may function at a distinct level in the viral lifecycle, at the point of glycosylated viral protein transport.

Product Categories/Family for anti-RSAD2 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 17974550
• NCBI GeneID: 91543
• UniProt Accession #: Q8WXG1; Q8WVI4
• Molecular Weight: 42,170 Da
• NCBI Official Full Name: viperin
• NCBI Official Synonym Full Names: radical S-adenosyl methionine domain containing 2
• NCBI Official Symbol: RSAD2
• NCBI Official Synonym Symbols: cig5; vig1; cig33; 2510004L01Rik
• NCBI Protein Information: radical S-adenosyl methionine domain-containing protein 2; viperin; cytomegalovirus-induced gene 5 protein; virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible
• UniProt Protein Name: Radical S-adenosyl methionine domain-containing protein 2
• Protein Family: Radical S-adenosyl methionine domain-containing protein
• UniProt Gene Name: RSAD2
• UniProt Synonym Gene Names: CIG5
• UniProt Entry Name: RSAD2_HUMAN

Uniprot Description

RSAD2: Interferon-inducible iron-sulfur (4FE-4S) cluster- binding antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Can inhibit a wide range of DNA and RNA viruses, including human cytomegalovirus (HCMV), hepatitis C virus (HCV), west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus, vesicular stomatitis virus (VSV), and human immunodeficiency virus (HIV-1). Displays antiviral activity against influenza A virus by inhibiting the budding of the virus from the plasma membrane by disturbing the lipid rafts. This is accomplished, at least in part, through binding and inhibition of the enzyme farnesyl diphospate synthase (FPPS), which is essential for the biosynthesis of isoprenoid-derived lipids. Promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating Lys-63'-linked ubiquitination of IRAK1. Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins. Belongs to the radical SAM superfamily. RSAD2 family.

Chromosomal Location of Human Ortholog: 2p25.2

Cellular Component: Golgi apparatus; mitochondrial outer membrane; endoplasmic reticulum membrane; mitochondrion; endoplasmic reticulum; mitochondrial inner membrane; lipid particle

Molecular Function: protein binding; protein self-association; 4 iron, 4 sulfur cluster binding; metal ion binding; catalytic activity

Biological Process: positive regulation of toll-like receptor 7 signaling pathway; negative regulation of protein secretion; ossification; negative regulation of viral genome replication; CD4-positive, alpha beta T cell differentiation; viral reproduction; metabolic process; response to virus; cytokine and chemokine mediated signaling pathway; positive regulation of toll-like receptor 9 signaling pathway; regulation of ossification; defense response to virus