Mouse VGLUT1 Monoclonal Antibody | anti-Slc17a7 antibody

VGLUT1 Antibody: ATTO 594

Cat. Num. AAA803638

• Gene Names: Slc17a7; BNPI; Vglut1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Synonyms: VGLUT1; Monoclonal Antibody; VGLUT1 Antibody: ATTO 594; BNPI; SLC17A1; SLC17A1/VGLUT1; Solute Carrier family 17 member 7; Vesicular glutamate transporter 1; VGLUT 1; Brain-specific Na(+)-dependent inorganic phosphate cotransporter; anti-Slc17a7 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: S28-9
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 560

• Applicable Applications for anti-Slc17a7 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: 1:1000 (WB)
• Conjugate: ATTO 594
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide.
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9. Tissue: spinal cord. Species: Mouse. Fixation: 4% PFA. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:500 for 16 hours at RT. Secondary Antibody: Alexa Fluor 555 Donkey Anti-Mouse (red) at 1:2000 for 2 hours at RT. Counterstain: NeuN neuronal stain (green). Magnification: 20X. Courtesy of: Leilei Wang, Ph.D. UT Southwestern Medical Center at Dallas.)

Western Blot (WB)

(Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:1000.)

Related Product Information for anti-Slc17a7 antibody

Background Info: Detects ~52kDa. No cross-reactivity against VGLUT2.

Scientific Background: VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).

Product Categories/Family for anti-Slc17a7 antibody

Transporters

References

1. Wojcik S.M., et al. (2004) PNAS. 101(18): 7158-7163. 2.   Shigeri Y., Seal R.P., Shimamoto K. (2004) Brain Res Rev. 45(3): 250-265.

NCBI and Uniprot Product Information

• NCBI GI #: 16758726
• NCBI GeneID: 116638
• NCBI Accession #: NP_446311.1
• NCBI GenBank Nucleotide #: NM_053859.2
• UniProt Accession #: Q62634; A9LRT0
• Molecular Weight: 64,333 Da
• NCBI Official Full Name: vesicular glutamate transporter 1
• NCBI Official Synonym Full Names: solute carrier family 17 (vesicular glutamate transporter), member 7
• NCBI Official Symbol: Slc17a7
• NCBI Official Synonym Symbols: BNPI; Vglut1
• NCBI Protein Information: vesicular glutamate transporter 1; solute carrier family 17 member 7; brain-specific Na-dependent inorganic phosphate cotransporter; brain-specific Na(+)-dependent inorganic phosphate cotransporter; solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
• UniProt Protein Name: Vesicular glutamate transporter 1
• Protein Family: Vesicular glutamate transporter
• UniProt Gene Name: Slc17a7
• UniProt Synonym Gene Names: Bnpi; Vglut1; VGluT1
• UniProt Entry Name: VGLU1_RAT

NCBI Description

a Na(+)-dependent inorganic phosphate cotransporter; may be involved in neuronal Na+ transport [RGD, Feb 2006]

Uniprot Description

SLC17A7: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.

Protein type: Transporter, SLC family; Transporter; Vesicle; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: synaptic vesicle; synaptic vesicle membrane; presynaptic active zone; integral to membrane; intracellular organelle; excitatory synapse; synapse; cell junction

Molecular Function: sodium:inorganic phosphate symporter activity; extracellular-glutamate-gated chloride channel activity; sodium-dependent phosphate transmembrane transporter activity; inorganic phosphate transmembrane transporter activity

Biological Process: synaptic transmission, glutamatergic; synaptic transmission; L-glutamate import; phosphate transport; long-term memory; neurotransmitter transport; sequestering of neurotransmitter; regulation of excitatory postsynaptic membrane potential