Rabbit anti-Human MRPS2 Polyclonal Antibody | anti-MRPS2 antibody

MRPS2, NT (MRPS2, 28S ribosomal protein S2, mitochondrial) (AP)

Cat. Num. AAA6322307

• Gene Names: MRPS2; S2mt; CGI-91; MRP-S2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MRPS2; Polyclonal Antibody; NT (MRPS2; 28S ribosomal protein S2; mitochondrial) (AP); mitochondrial; anti-MRPS2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-MRPS2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MRPS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-71 amino acids from the N-terminal region of human MRPS2.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MRPS2 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MRPS2 antibody detected the MRPS2 protein (arrow).)

Related Product Information for anti-MRPS2 antibody

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S2 family.

Product Categories/Family for anti-MRPS2 antibody

Antibodies; Mitochondrial Proteins

References

Zhang, Z., et al. Genomics 81(5):468-480(2003). Kenmochi, N., et al. Genomics 77 (1-2), 65-70 (2001): Suzuki, T., et al. J. Biol. Chem. 276(35):33181-33195(2001). Cavdar Koc, E., et al. J. Biol. Chem. 276(22):19363-19374(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 7705805
• NCBI GeneID: 51116
• NCBI Accession #: NP_057118.1
• NCBI GenBank Nucleotide #: NM_016034.4
• UniProt Accession #: Q9Y399; Q5T899; Q9BSQ4
• Molecular Weight: 33,249 Da
• NCBI Official Full Name: 28S ribosomal protein S2, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein S2
• NCBI Official Symbol: MRPS2
• NCBI Official Synonym Symbols: S2mt; CGI-91; MRP-S2
• NCBI Protein Information: 28S ribosomal protein S2, mitochondrial
• UniProt Protein Name: 28S ribosomal protein S2, mitochondrial
• Protein Family: 28S ribosomal protein
• UniProt Gene Name: MRPS2
• UniProt Synonym Gene Names: MRP-S2; S2mt
• UniProt Entry Name: RT02_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S2 family. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

MRPS2: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S2 family. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]

Protein type: Ribosomal; Translation; Mitochondrial

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: mitochondrion; mitochondrial small ribosomal subunit

Molecular Function: structural constituent of ribosome

Biological Process: translation