Mouse anti-Human VAP-1 Monoclonal Antibody | anti-VAP-1 antibody

VAP-1, Human, mAb 174-5

Cat. Num. AAA584303

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: VAP-1; Monoclonal Antibody; Human; mAb 174-5; membrane primary amine oxidase; AOC3; SSAO; anti-VAP-1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 20

• Applicable Applications for anti-VAP-1 antibody: F, Flow Cytometry (FC/FACS), FS, Immunofluorescence (IF)
• Application Notes: Application Notes: FC: Antibody 174-5 stains Ax cells stably transfected with VAP-1 cDNA.. As negative control mock transfected cells were used.(Ref.1); Application Use: For immunohistochemistry, and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.
• Immunogen: Purified vessels from human peripheral lymph nodes (ref 1)
• Positive Control: Ax cells stably transfected with VAP-1 cDNA. (Ref. 1)
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Related Product Information for anti-VAP-1 antibody

Product Description: The monoclonal antibody 174-5 recognises human Vascular Adhesion Protein-1 (VAP-1), which- is a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. It contains- a short N-terminal cytoplasmic tail, a single membrane-spanning domain and a large extracellular part.- A soluble form of VAP-1 (sVAP-1) has been described, which presumably results from the proteolytic cleavage of membrane-bound VAP-1. Structurally VAP-1 belongs to enzymes called semicarbamizide-sensitive amine oxidases, which contain copper as a cofactor. These enzymes deaminate primary amines in a reaction producing hydrogen peroxide, aldehyde, and ammonia.
VAP-1 is present in endothelial cells, smooth muscle cells, adipocytes, and in follicular dendritic cells. In endothelial cells the majority of VAP-1 is stored within intracellular granules and translocated to the surface upon inflammation where it regulates leukocyte tissue infiltration. Furthermore, the end-products formed by VAP-1 can also regulate leukocyte migration by signaling effects, have insulin-like effects in energy metabolism, and can cause vascular damage by direct cytotoxicity. Elevated sVAP-1serum- levels have been described in several inflammatory- diseases as well as colorectal cancer. Moreover, diminished insulin secretion appears to increase the concentration of soluble VAP-1 in plasma. Therefore, VAP-1 might be an interesting marker as well therapeutic- target for modulating inflammation.The monoclonal antibody 174-5 has been shown to cross-react with rat VAP-1 and to inhibit lymphocyte infiltration in rat liver allograft rejection.

References

1. Martelius, T et al; Blockade of vascular adhesion protein-1 inhibits lymphocyte infiltration in rat liver allograft rejection. Am J Path 2004, 165: 1993. 2. Autio, A et al; PET imaging of inflammation and adenocarcinoma zenografts using vascular adhesion protein 1 targeting peptide 68Ga-DOTAVAP-P1: comparison with 8F-FDG, Eur J Nucl Med Mol Imaging 2010, 37:1918.

NCBI and Uniprot Product Information

• NCBI GI #: 255615
• NCBI Official Full Name: vascular adhesion protein-1, VAP-1=90 kda lymphocyte binding endothelial cell adhesion molecule {N-terminal}, partial
• Protein Family: Vesicle-associated protein