Human PCNA Associated Factor 15kDa (PAF) ELISA Kit | PAF elisa kit

Human PCNA Associated Factor 15kDa (PAF) ELISA Kit

Cat. Num. AAA456903

• Gene Names: PCLAF; L5; PAF; OEATC; PAF15; OEATC1; p15PAF; NS5ATP9; OEATC-1; p15/PAF; KIAA0101; p15(PAF)
• Reactivity: Human
• Synonyms: PCNA Associated Factor 15kDa (PAF); Human PCNA Associated Factor 15kDa (PAF) ELISA Kit; p15(PAF); L5; OEATC1; NS5ATP9; Overexpressed In Anaplastic Thyroid Carcinoma 1; Hepatitis C virus NS5A-transactivated protein 9; PAF elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PAF. No significant cross-reactivity or interference between PAF and analogues was observed.
• Sequence Length: 65

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.781-50ng/mL
• Sensitivity: <0.29ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PAF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PAF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PAF elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PAF in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PAF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PAF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PAF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PAF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 71773819
• NCBI GeneID: 9768
• NCBI Accession #: NP_001025160.1
• NCBI GenBank Nucleotide #: NM_001029989.2
• UniProt Accession #: Q15004; A6NNU5; A8K3Y3; G9G694; G9G696
• Molecular Weight: 7,221 Da
• NCBI Official Full Name: PCNA-associated factor isoform 2
• NCBI Official Synonym Full Names: PCNA clamp associated factor
• NCBI Official Symbol: PCLAF
• NCBI Official Synonym Symbols: L5; PAF; OEATC; PAF15; OEATC1; p15PAF; NS5ATP9; OEATC-1; p15/PAF; KIAA0101; p15(PAF)
• NCBI Protein Information: PCNA-associated factor
• UniProt Protein Name: PCNA-associated factor
• Protein Family: PCNA-associated factor
• UniProt Gene Name: PCLAF
• UniProt Synonym Gene Names: HCV NS5A-transactivated protein 9; OEATC-1; PAF15; p15PAF

Uniprot Description

PAF: May be involved in protection of cells from UV-induced cell death.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: centrosome; nucleoplasm; nucleus; perinuclear region of cytoplasm

Molecular Function: chromatin binding; protein binding

Biological Process: bypass DNA synthesis; cellular response to DNA damage stimulus; centrosome cycle; DNA replication; regulation of cell cycle; response to UV