Rat anti-Mouse VAP-1 Monoclonal Antibody | anti-vap-1 antibody

VAP-1 (Vascular Adhesion Protein 1, VAP1, Amine Oxidase Copper-containing 3 (Vascular Adhesion Protein 1), AOC3, Copper Amine Oxidase, HPAO, Membrane Copper Amine Oxidase, Semicarbazide-sensitive Amine Oxidase, SSAO, VP97)

Cat. Num. AAA608270

• Reactivity: Mouse
• Applications: Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: VAP-1; Monoclonal Antibody; VAP-1 (Vascular Adhesion Protein 1; VAP1; Amine Oxidase Copper-containing 3 (Vascular Adhesion Protein 1); AOC3; Copper Amine Oxidase; HPAO; Membrane Copper Amine Oxidase; Semicarbazide-sensitive Amine Oxidase; SSAO; VP97); Anti -VAP-1 (Vascular Adhesion Protein 1; anti-vap-1 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 9G298
• Specificity: Recognizes mouse Vascular Adhesion Protein-1 (VAP-1), also known as AOC3, a heavily sialylated homodimeric glycoprotein of 180kD. Inhibits migration of granulocytes and monocytes in acute models of inflammation.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA.

• Applicable Applications for anti-vap-1 antibody: Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunohistochemistry, Immunoprecipitation, Immunofluorescence, Flow Cytometry and Inhibition of biological activity.; Dilution: Immunohistochemistry (frozen): 1:50. Tissue sections were fixed in acetone and incubated with antibody for 20 min at RT.; Flow Cytometry: 1:50. Stains the extracellular domain of mouse VAP-1 in CHO cells transfected with mouse VAP-1 domain.
• Immunogen: Vessels from mouse lymph nodes.
• Positive Control: Mouse VAP-1-transfected CHO cells
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunofluorescence (IF)

(Vascular staining of VAP-1 in mouse spleen. Immunofluorescence staining of a frozen section using MBS608270.)

Related Product Information for anti-vap-1 antibody

VAP-1 is present in endothelial cells, smooth muscle cells, adipocytes, and in follicular dendritic cells. The majority of VAP-1 is stored within intracellular granules and translocated to the endothelial surface on inflammation. Structurally it belongs to enzymes called semicarbamizide-sensitive amine oxidases. These enzymes deaminate primary amines in a reaction producing hydrogen peroxide, aldehyde, and ammonia. VAP-1 possesses both adhesive and enzymatic properties in vitro. VAP-1 mediates leukocyte trafficking to sites of inflammation.

Product Categories/Family for anti-vap-1 antibody

Antibodies; Abs to Enzymes; Oxidase

NCBI and Uniprot Product Information

• NCBI GI #: 71987263
• NCBI GeneID: 181768
• NCBI Accession #: NP_001024553.1
• NCBI GenBank Nucleotide #: NM_001029382.1
• UniProt Accession #: Q8MQ79
• Molecular Weight: 46,224 Da
• NCBI Official Full Name: Venom-Allergen-like Protein family member (vap-1)
• NCBI Official Symbol: vap-1
• NCBI Protein Information: Venom-Allergen-like Protein family member (vap-1)
• UniProt Protein Name: Venom-allergen-like protein protein 1, isoform b
• Protein Family: Vesicle-associated protein
• UniProt Gene Name: vap-1
• UniProt Entry Name: Q8MQ79_CAEEL

Uniprot Description

Enzyme and pathway databasesReactome - a knowledgebase of biological pathways and processes<br/><a href='/database/88'>More..</a>ReactomeiR-CEL-6798695. Neutrophil degranulation.