Rabbit AOC3 Polyclonal Antibody | anti-AOC3 antibody

AOC3 Antibody

Cat. Num. AAA9607401

• Gene Names: AOC3; HPAO; SSAO; VAP1; VAP-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: AOC3; Polyclonal Antibody; AOC3 Antibody; Amine oxidase; copper containing 3 (vascular adhesion protein 1); AOC3_HUMAN; Copper amine oxidase; HPAO; Membrane copper amine oxidase; Membrane primary amine oxidase; Semicarbazide sensitive amine oxidase; Semicarbazide-sensitive amine oxidase; SSAO; VAP-1; Vascular adhesion protein 1; VP97; anti-AOC3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: AOC3 antibody detects endogenous levels of total AOC3
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 634

• Applicable Applications for anti-AOC3 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human AOC3
• Subcellular Location: Membrane.
• Tissue Specificity: Strongly expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. Also highly expressed in appendix, lung and small intestine. Expressed also in adipose tissue, in bone marrow, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, spleen and testis. Isoform 2 seems to be the predominant transcript in fetal kidneys, fetal cartilage and fetal tonsils. The highest relative expression of isoform 2 occurs in skeletal muscle, heart, pancreas, kidney, and lung.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of A549 whole cell lysates, using AOC3 Antibody. The lane on the left is treated with the antigen-specific peptide.)

Immunohistochemistry (IHC)

(MBS9607401 at 1/100 staining Human lymph tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Related Product Information for anti-AOC3 antibody

Description: This gene encodes a member of the semicarbazide-sensitive amine oxidase family. Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The encoded protein is localized to the cell surface, has adhesive properties as well as monoamine oxidase activity, and may be involved in leukocyte trafficking. Alterations in levels of the encoded protein may be associated with many diseases, including diabetes mellitus. A pseudogene of this gene has been described and is located approximately 9-kb downstream on the same chromosome. Alternative splicing results in multiple transcript variants.
Function: Cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has semicarbazide-sensitive (SSAO) monoamine oxidase activity. May play a role in adipogenesis.
Subunit Structure: Homodimer; disulfide-linked. Can heterodimerize with isoform 2 leading to reduced surface expression. Forms a heterodimer with AOC2.
Post-translational Modifications: Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue. N-and O-glycosylated.
Similarity: Belongs to the copper/topaquinone oxidase family.

NCBI and Uniprot Product Information

• NCBI GI #: 480306390
• NCBI GeneID: 8639
• NCBI Accession #: NP_001264660.1
• NCBI GenBank Nucleotide #: NM_001277731.1
• UniProt Accession #: Q16853; Q45F94; B2RCI5; K7ESB3; L0L8N9
• Molecular Weight: Observed: 84 kDa; Predicted: 85 kDa
• NCBI Official Full Name: membrane primary amine oxidase isoform 2
• NCBI Official Synonym Full Names: amine oxidase, copper containing 3
• NCBI Official Symbol: AOC3
• NCBI Official Synonym Symbols: HPAO; SSAO; VAP1; VAP-1
• NCBI Protein Information: membrane primary amine oxidase
• UniProt Protein Name: Membrane primary amine oxidase
• Protein Family: Allene oxide cyclase
• UniProt Gene Name: AOC3
• UniProt Synonym Gene Names: VAP1; SSAO; VAP-1

NCBI Description

This gene encodes a member of the semicarbazide-sensitive amine oxidase family. Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The encoded protein is localized to the cell surface, has adhesive properties as well as monoamine oxidase activity, and may be involved in leukocyte trafficking. Alterations in levels of the encoded protein may be associated with many diseases, including diabetes mellitus. A pseudogene of this gene has been described and is located approximately 9-kb downstream on the same chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013]

Uniprot Description

Cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has semicarbazide-sensitive (SSAO) monoamine oxidase activity. May play a role in adipogenesis.