Western Blot (WB)
(Anti-DCUN1D2 rabbit monoclonal antibody at 1:500 dilutionLane A: HCT116 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:30 kDaObserved band size:30 kDa)
Rabbit anti-Human DCUN1D2 Monoclonal Antibody | anti-DCUN1D2 antibody
Recombinant Anti-DCUN1D2 Antibody, Rabbit Monoclonal
Gene Names
DCUN1D2; C13orf17
Reactivity
Human
Applications
Western Blot, ELISA, Immunoprecipitation
Purity
Protein A
Synonyms
DCUN1D2; Monoclonal Antibody; Recombinant Anti-DCUN1D2 Antibody; Rabbit Monoclonal; DCUN1D2/DCN1-like protein 2 Antibody; Rabbit MAb; DCN1; defective in cullin neddylation 1; domain containing 2; Anti-C13orf17 Antibody; anti-DCUN1D2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
001
Specificity
Human DCUN1D2/DCN1-like protein 2
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-DCUN1D2 antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
IP: 1-4uL/mg of lysate
ELISA: 1:5000-1:10,000
IP: 1-4uL/mg of lysate
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human DCUN1D2/DCN1-like protein 2 Protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human DCUN1D2/DCN1-like protein 2 (rh DCUN1D2/DCN1-like protein 2).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-DCUN1D2 rabbit monoclonal antibody at 1:500 dilutionLane A: HCT116 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:30 kDaObserved band size:30 kDa)
Western Blot (WB)
(Anti-DCUN1D2 rabbit monoclonal antibody at 1:500 dilutionLane A: HCT116 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:30 kDaObserved band size:30 kDa)
Immunoprecipitation (IP)
(DCUN1D2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg A549 Whole Cell LysateLane C:0.5 mg HuT78 Whole Cell Lysate2 uL anti-DCUN1D2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DCUN1D2 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size: 30 kDa)
Immunoprecipitation (IP)
(DCUN1D2 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg A549 Whole Cell LysateLane C:0.5 mg HuT78 Whole Cell Lysate2 uL anti-DCUN1D2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DCUN1D2 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size: 30 kDa)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,467 Da
NCBI Official Full Name
DCN1-like protein 2
NCBI Official Synonym Full Names
DCN1, defective in cullin neddylation 1, domain containing 2
NCBI Official Symbol
DCUN1D2
NCBI Official Synonym Symbols
C13orf17
NCBI Protein Information
DCN1-like protein 2; DCUN1 domain-containing protein 2; defective in cullin neddylation protein 1-like protein 2
UniProt Protein Name
DCN1-like protein 2
Protein Family
UniProt Gene Name
DCUN1D2
UniProt Synonym Gene Names
C13orf17; DCUN1L2
UniProt Entry Name
DCNL2_HUMAN
Uniprot Description
DCUN1D2: May contribute to neddylation of cullin components of SCF-type E3 ubiquitin ligase complexes. Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 13q34
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Product Notes
The DCUN1D2 dcun1d2 (Catalog #AAA8107555) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-DCUN1D2 Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DCUN1D2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the DCUN1D2 dcun1d2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DCUN1D2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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