Rat anti-Mouse Uteroglobin Related Protein 1 Monoclonal Antibody | anti-SCGB3A2 antibody
Uteroglobin Related Protein 1 (UGRP1, Secretoglobin Family 3A Member 2, Pneumo Secretory Protein 1, PnSP-1)
Gene Names
SCGB3A2; LU103; PNSP1; UGRP1; SCGB3A2
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Uteroglobin Related Protein 1; Monoclonal Antibody; Uteroglobin Related Protein 1 (UGRP1; Secretoglobin Family 3A Member 2; Pneumo Secretory Protein 1; PnSP-1); Anti -Uteroglobin Related Protein 1 (UGRP1; anti-SCGB3A2 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
7H174
Specificity
Selected for its ability to detect rmUGRP1 in direct ELISAs and Western Blots. In these formats, this antibody shows no crossreactivity with rhUGRP1.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.2ml is used, the concentration will be 0.5mg/ml.
Applicable Applications for anti-SCGB3A2 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Western Blot: This antibody can be used at 1-2ug/ml with the appropriate secondary reagents to detect mouse UGRP1. Using a colorimetric detection system, the detection limit for rmUGRP1 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse UGRP1. The detection limit for rmUGRP1 is approximately 25 ng/well., Optimal dilutions should be determined by each laboratory for each application.
Immunogen
E. coli-derived rmUGRP1
Hybridoma
Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, E,coli-derived, recombinant mouse uteroglobin-related protein-1 (rmUGRP1; aa 22-139: Accession #Q920H1).
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-SCGB3A2 antibody
UGRP1 (also secretglobin gene family 3A2/SCGB3A2) is an 18 kD, secreted nonglycosylated polypeptide that is a member of the uteroglobulin family, UGRP subfamily. Mouse UGRP1(A) circulates as a 37 kD homodimer. Its mature form is 70 amino acids in length. There are two large alternate splice forms termed B and C. UGRP1B is a 113 aa secreted homodimer that shows a 22 aa insert between aa's 84 and 85 of the short form. UGRP1C theoretically shows a 55 aa substitution for the C-terminal seven aa of the short form. Its existence is unclear. Mature mouse UGRP1A is 86% and 81% aa identical to rat and human UGRP1, respectively.
Product Categories/Family for anti-SCGB3A2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
10,161 Da
NCBI Official Full Name
uteroglobin related protein 1
NCBI Official Synonym Full Names
secretoglobin, family 3A, member 2
NCBI Official Symbol
SCGB3A2
NCBI Official Synonym Symbols
LU103; PNSP1; UGRP1; SCGB3A2
NCBI Protein Information
secretoglobin family 3A member 2; pnSP-1; pneumo secretory protein 1; uteroglobin-related protein 1
UniProt Protein Name
Secretoglobin, family 3A, member 2
Protein Family
UniProt Gene Name
UGRP1
UniProt Synonym Gene Names
SCGB3A2
UniProt Entry Name
Q2L6B3_HUMAN
NCBI Description
The protein encoded by this gene is a secreted lung surfactant protein and a downstream target of thyroid transcription factor. A single nucleotide polymorphism in the promoter of this gene results in susceptibility to asthma.
Uniprot Description
SCGB3A2: is a secreted lung surfactant protein and a downstream target of thyroid transcription factor. A single nucleotide polymorphism in the promoter of this gene results in susceptibility to asthma.[provided by RefSeq, Mar 2010]
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 5q32
Cellular Component: extracellular region
Biological Process: receptor-mediated endocytosis
Disease: Asthma, Susceptibility To
Research Articles on SCGB3A2
1. association was detected between SCGB3A2 and U.K. Caucasian Graves' disease subjects but the size of effect was smaller than that seen in the Oriental population (odds ratio = 1.28-1.73).
2. SCGB3A2 gene may contribute to Graves' disease susceptibility.
3. plasma UGRP1 levels were associated with the G-112A UGRP1 gene promoter polymorphism and the severity of asthma
4. Observational study of gene-disease association. (HuGE Navigator)
5. results suggest that the -112G/A polymorphism does not play a significant role in the genetic predisposition of the UGRP1 gene in atopic asthma in the Sicilian population
6. polymorphism in the human UGRP1 gene promoter that regulates transcription is associated with an increased risk of asthma
7. results demonstrate that interleukin-10 induces uteroglobin related protein 1(UGRP1) gene expression in lung epithelial cells through transcription factor T/EBP/NKX2.1-dependent pathway
8. By in situ hybridization, UGRP1 is found to be expressed by lung Clara-like cells in the bronchial epithelium and up-regulated in lung epithelium of a patient with cystic fibrosis.
2. SCGB3A2 gene may contribute to Graves' disease susceptibility.
3. plasma UGRP1 levels were associated with the G-112A UGRP1 gene promoter polymorphism and the severity of asthma
4. Observational study of gene-disease association. (HuGE Navigator)
5. results suggest that the -112G/A polymorphism does not play a significant role in the genetic predisposition of the UGRP1 gene in atopic asthma in the Sicilian population
6. polymorphism in the human UGRP1 gene promoter that regulates transcription is associated with an increased risk of asthma
7. results demonstrate that interleukin-10 induces uteroglobin related protein 1(UGRP1) gene expression in lung epithelial cells through transcription factor T/EBP/NKX2.1-dependent pathway
8. By in situ hybridization, UGRP1 is found to be expressed by lung Clara-like cells in the bronchial epithelium and up-regulated in lung epithelium of a patient with cystic fibrosis.
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Product Notes
The SCGB3A2 ugrp1 (Catalog #AAA601166) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Uteroglobin Related Protein 1 (UGRP1, Secretoglobin Family 3A Member 2, Pneumo Secretory Protein 1, PnSP-1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Uteroglobin Related Protein 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Western Blot: This antibody can be used at 1-2ug/ml with the appropriate secondary reagents to detect mouse UGRP1. Using a colorimetric detection system, the detection limit for rmUGRP1 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse UGRP1. The detection limit for rmUGRP1 is approximately 25 ng/well., Optimal dilutions should be determined by each laboratory for each application. Researchers should empirically determine the suitability of the SCGB3A2 ugrp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Uteroglobin Related Protein 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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