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Western Blot (WB) (Western blot analysis of USP7 on 293T cell using anti-USP7 antibody at 1/500 dilution.)

Rabbit USP7 Monoclonal Antibody | anti-USP7 antibody

USP7 Antibody

Gene Names
USP7; TEF1; HAUSP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
USP7; Monoclonal Antibody; USP7 Antibody; Deubiquitinating enzyme 7 antibody; HAUSP antibody; Herpes virus associated ubiquitin specific protease antibody; Herpesvirus-associated ubiquitin-specific protease antibody; TEF 1 antibody; tef-1 antibody; TEF1 antibody; Ubiquitin carboxyl terminal hydrolase 7 antibody; Ubiquitin carboxyl-terminal hydrolase 7 antibody; Ubiquitin specific peptidase 7 (herpes virus associated) antibody; Ubiquitin specific peptidase 7 antibody; Ubiquitin specific peptidase 7 herpes virus associated antibody; Ubiquitin specific processing protease 7 antibody; Ubiquitin specific protease 7 (herpes virus associated) antibody; Ubiquitin specific protease 7 antibody; Ubiquitin specific protease 7 herpes virus associated antibody; Ubiquitin thioesterase 7 antibody; Ubiquitin thiolesterase 7 antibody; Ubiquitin-specific-processing protease 7 antibody; UBP 7 antibody; UBP-7 antibody; UBP7 antibody; UBP7_HUMAN antibody; USP 7 antibody; usp-7 antibody; Usp7 antibody; VMW110-ASSOCIATED PROTEIN; 135-KD antibody; anti-USP7 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JB80-36
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-USP7 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:1000
IHC: 1:50
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein within human USP7 aa 100-200.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of USP7 on 293T cell using anti-USP7 antibody at 1/500 dilution.)

Western Blot (WB) (Western blot analysis of USP7 on 293T cell using anti-USP7 antibody at 1/500 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-USP7 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-USP7 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-USP7 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-USP7 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-USP7 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-USP7 antibody. Counter stained with hematoxylin.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of PC-3M cells with USP7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of PC-3M cells with USP7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Related Product Information for anti-USP7 antibody
HAUSP (herpesvirus-associated ubiquitin-specific protease, USP7) is a ubiquitin-specific protease. HAUSP localizes predominantly to the nucleus, in a TD-dependent manner, where it associates with ND10. ND10 are small nuclear structures implicated in a variety of cellular processes including response to stress and interferons, oncogenesis, and viral infection. HAUSP binds strongly to Vmw110, a herpesvirus regulatory protein which has the ability to disrupt ND10. HAUSP, a novel p53-interacting protein, functions to deubiquitinize p53 in an important pathway for p53 stabilization. HAUSP strongly stabilizes p53 even in the presence of excess Mdm2, and also induces p53-dependent cell growth repression and apoptosis. The HAUSP protein is distributed in the nucleus in a micropunctate pattern with a limited number of larger discrete foci, some of which co-localize with PML in ND10. The gene encoding HAUSP maps to human chromosome band 16p13.2.
Product Categories/Family for anti-USP7 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
1102
NCBI Official Full Name
Ubiquitin carboxyl-terminal hydrolase 7
NCBI Official Synonym Full Names
ubiquitin specific peptidase 7 (herpes virus-associated)
NCBI Official Symbol
USP7
NCBI Official Synonym Symbols
TEF1; HAUSP
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 7; ubiquitin thioesterase 7; deubiquitinating enzyme 7; ubiquitin-specific-processing protease 7; herpesvirus-associated ubiquitin-specific protease; Herpes virus-associated ubiquitin-specific protease; ubiquitin spec
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 7
UniProt Gene Name
USP7
UniProt Synonym Gene Names
HAUSP
UniProt Entry Name
UBP7_HUMAN

Uniprot Description

USP7: an enzyme that removes ubiquitin from its protein substrates. Binds to the herpes virus protein VMW110 that may therefore modulate its substrate specificity or activity to stabilize viral proteins. Regulates MDM2 and has a dynamic role in the p53-MDM2 pathway. Is reported to deubiquitinate and stabilize p53.

Protein type: Ubiquitin-specific protease; Protease; EC 3.4.19.12; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nuclear body; PML body; cytosol; nucleus

Molecular Function: protein C-terminus binding; protein binding; p53 binding; cysteine-type endopeptidase activity; ubiquitin protein ligase binding; ubiquitin-specific protease activity; transcription factor binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; maintenance of DNA methylation; viral reproduction; regulation of transcription factor activity; inhibition of NF-kappaB transcription factor; transcription-coupled nucleotide-excision repair; multicellular organismal development; histone deubiquitination

Research Articles on USP7

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Product Notes

The USP7 usp7 (Catalog #AAA9436573) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The USP7 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's USP7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:1000 IHC: 1:50 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the USP7 usp7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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