Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Tyrosine Hydroxylase (TH) Standard Curve.)
Rat Tyrosine Hydroxylase (TH) ELISA Kit | TH elisa kit
Rat Tyrosine Hydroxylase (TH) ELISA Kit
Gene Names
Th; The
Reactivity
Rat
Synonyms
Tyrosine Hydroxylase (TH); Rat Tyrosine Hydroxylase (TH) ELISA Kit; TH; DYT14; DYT5b; TYH; dystonia 14; tyrosine 3-monooxygenase; TH elisa kit
Reactivity
Rat
Specificity
Rat Tyrosine Hydroxylase (TH) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TH. No significant cross-reactivity or interference between Rat TH and analogues was observed.
Sequence Length
498
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Tyrosine Hydroxylase (TH) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Tyrosine Hydroxylase (TH) Standard Curve.)
Related Product Information for TH elisa kit
Description: This Rat Tyrosine Hydroxylase (TH) ELISA Kit employs a two-site sandwich ELISA to quantitate TH in samples. An antibody specific for TH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The protein encoded by Tyrosine Hydroxylase gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons.
Background: The protein encoded by Tyrosine Hydroxylase gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tyrosine 3-monooxygenase
NCBI Official Synonym Full Names
tyrosine hydroxylase
NCBI Official Symbol
Th
NCBI Official Synonym Symbols
The
NCBI Protein Information
tyrosine 3-monooxygenase
UniProt Protein Name
Tyrosine 3-monooxygenase
UniProt Gene Name
Th
UniProt Entry Name
TY3H_RAT
NCBI Description
catalyzes the rate limiting step in catecholamine biosynthesis; may be involved in response to hypoxia [RGD, Feb 2006]
Research Articles on TH
Similar Products
Product Notes
The Rat TH th (Catalog #AAA9711829) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711829 ELISA Kit recognizes Rat TH. It is sometimes possible for the material contained within the vial of "Tyrosine Hydroxylase (TH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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