Immunofluorescence (IF)
(Immunofluorescence staining of Human USP7 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human USP7 monoclonal antibody (1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody(green). Positive staining was localized to cytoplasm and nucleus.)
Mouse anti-Human USP7 Monoclonal Antibody | anti-USP7 antibody
Anti-USP7 Antibody, Mouse Monoclonal
ICC/IF: 1:20-1:100
FC/FACS/FCM: 1:25-1:100
Immunofluorescence (IF)
(Immunofluorescence staining of Human USP7 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human USP7 monoclonal antibody (1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody(green). Positive staining was localized to cytoplasm and nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of Human USP7 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human USP7 monoclonal antibody (1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody(green). Positive staining was localized to cytoplasm and nucleus.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human USP7 expression on Jurkat cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human USP7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human USP7 expression on Jurkat cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human USP7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
NCBI and Uniprot Product Information
Uniprot Description
USP7: an enzyme that removes ubiquitin from its protein substrates. Binds to the herpes virus protein VMW110 that may therefore modulate its substrate specificity or activity to stabilize viral proteins. Regulates MDM2 and has a dynamic role in the p53-MDM2 pathway. Is reported to deubiquitinate and stabilize p53.
Protein type: Ubiquitin-specific protease; Protease; EC 3.4.19.12; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: nuclear body; PML body; cytosol; nucleus
Molecular Function: protein C-terminus binding; protein binding; p53 binding; cysteine-type endopeptidase activity; ubiquitin protein ligase binding; ubiquitin-specific protease activity; transcription factor binding
Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; maintenance of DNA methylation; viral reproduction; regulation of transcription factor activity; inhibition of NF-kappaB transcription factor; transcription-coupled nucleotide-excision repair; multicellular organismal development; histone deubiquitination
Research Articles on USP7
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Product Notes
The USP7 usp7 (Catalog #AAA8105617) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-USP7 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's USP7 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF). ELISA: 1:1000-1:2000 ICC/IF: 1:20-1:100 FC/FACS/FCM: 1:25-1:100. Researchers should empirically determine the suitability of the USP7 usp7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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