Rabbit HAUSP / USP7 Polyclonal Antibody | anti-HAUSP antibody

Rabbit Polyclonal to Human HAUSP / USP7

Cat. Num. AAA243015

• Gene Names: USP7; TEF1; HAUSP
• Reactivity: Chimpanzee, Chicken, Dog, Horse, Human, Macaque, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein A Purified
• Synonyms: HAUSP / USP7; Polyclonal Antibody; Rabbit Polyclonal to Human HAUSP / USP7; Anti-HAUSP / USP7 Antibody (aa1050-1100) IHC-plus; USP7; Deubiquitinating enzyme 7; HAUSP; TEF1; Ubiquitin specific protease 7; Ubiquitin thioesterase 7; Human HAUSP; anti-HAUSP antibody

• Host: Rabbit
• Reactivity: Chimpanzee, Chicken, Dog, Horse, Human, Macaque, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Human USP7
• Purity/Purification: Protein A Purified
• Form/Format: PBS, 0.05% BSA, 0.05% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 1102

• Applicable Applications for anti-HAUSP antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (1 - 2 ug/ml)
• Target Species: Human
• Immunogen Description: A portion of amino acids 1050-1100 of human USP7 protein was used as the immunogen.
• Immunogen Type: Synthetic peptide
• Immunogen: HAUSP / USP7 antibody was raised against a portion of amino acids 1050-1100 of human USP7 protein was used as the immunogen.
• Antigen Modification: aa1050-1100
• Preparation and Storage: Store at 4 degree C for short term applications. For long term storage, aliquot and store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-USP7 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-USP7 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western blot of USP7 protein in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide using at 1.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.)

Related Product Information for anti-HAUSP antibody

Ubiquitin-specific protease 7 (USP7) as a cysteine protease removes ubiquitin from conjugates and has been shown to regulate the levels of tumor suppressor protein p53 by deubiquitinating MDM2 (a ubiquitin ligase). USP7 is also known as herpes virus-associated ubiquitin-specific protease (HAUSP) because it associates with Herpes simplex virus type I immediate-early protein Vmw110.

Product Categories/Family for anti-HAUSP antibody

Family: Protease; Subfamily: Cysteine C19

NCBI and Uniprot Product Information

• NCBI GI #: 150378533
• NCBI GeneID: 7874
• NCBI Accession #: NP_003461.2
• NCBI GenBank Nucleotide #: NM_003470.2
• UniProt Accession #: Q93009; A6NMY8; B7Z815; H0Y3G8
• Molecular Weight: – Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 7 isoform 1
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 7 (herpes virus-associated)
• NCBI Official Symbol: USP7
• NCBI Official Synonym Symbols: TEF1; HAUSP
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 7; ubiquitin thioesterase 7; deubiquitinating enzyme 7; ubiquitin-specific-processing protease 7; herpesvirus-associated ubiquitin-specific protease; Herpes virus-associated ubiquitin-specific protease; ubiquitin specific protease 7 (herpes virus-associated)
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 7
• UniProt Gene Name: USP7
• UniProt Synonym Gene Names: HAUSP
• UniProt Entry Name: UBP7_HUMAN

Uniprot Description

USP7: an enzyme that removes ubiquitin from its protein substrates. Binds to the herpes virus protein VMW110 that may therefore modulate its substrate specificity or activity to stabilize viral proteins. Regulates MDM2 and has a dynamic role in the p53-MDM2 pathway. Is reported to deubiquitinate and stabilize p53.

Protein type: EC 3.4.19.12; Ubiquitin-specific protease; Ubiquitin conjugating system; Protease

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nuclear body; PML body; nucleus; cytosol

Molecular Function: protein C-terminus binding; protein binding; p53 binding; ubiquitin protein ligase binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; transcription factor binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; viral reproduction; regulation of transcription factor activity; maintenance of DNA methylation; inhibition of NF-kappaB transcription factor; transcription-coupled nucleotide-excision repair; multicellular organismal development; histone deubiquitination