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Western Blot (WB) (Western Blot: Sample: Recombinant UCN2, Human.)

Mouse anti-Human Urocortin 2 (UCN2) Monoclonal Antibody | anti-UCN2 antibody

Monoclonal Antibody to Urocortin 2 (UCN2)

Gene Names
UCN2; UR; SRP; URP; UCNI; UCN-II
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Urocortin 2 (UCN2); Monoclonal Antibody; Monoclonal Antibody to Urocortin 2 (UCN2); anti-UCN2 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a Kappa
Clone Number
C2
Specificity
The antibody is a mouse monoclonal antibody raised against UCN2. It has been selected for its ability to recognize UCN2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGS-V PVTPIPTFQL RPQNSPQTTP RPAASESPSA APTWPWAAQS
Applicable Applications for anti-UCN2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant UCN2 (Val20~Cys112) expressed in E.coli.
Cross Reactivity
Human
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2066857
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant UCN2, Human.)

Western Blot (WB) (Western Blot: Sample: Recombinant UCN2, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Liver Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Liver Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12,146 Da
NCBI Official Full Name
urocortin-2 preproprotein
NCBI Official Synonym Full Names
urocortin 2
NCBI Official Symbol
UCN2
NCBI Official Synonym Symbols
UR; SRP; URP; UCNI; UCN-II
NCBI Protein Information
urocortin-2
UniProt Protein Name
Urocortin-2
Protein Family
UniProt Gene Name
UCN2
UniProt Synonym Gene Names
SRP; URP; Ucn II

NCBI Description

This gene is a member of the sauvagine/corticotropin-releasing factor/urotensin I family. It is structurally related to the corticotropin-releasing factor (CRF) gene and the encoded product is an endogenous ligand for CRF type 2 receptors. In the brain it may be responsible for the effects of stress on appetite. In spite of the gene family name similarity, the product of this gene has no sequence similarity to urotensin II. [provided by RefSeq, Jul 2008]

Uniprot Description

UCN2: Suppresses food intake, delays gastric emptying and decreases heat-induced edema. Might represent an endogenous ligand for maintaining homeostasis after stress. Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: extracellular region; extracellular space; intracellular

Molecular Function: corticotropin-releasing hormone receptor 2 binding; G-protein coupled receptor binding; hormone activity; hormone binding; receptor binding

Biological Process: adenylate cyclase-activating G-protein coupled receptor signaling pathway; cAMP biosynthetic process; cellular response to nutrient levels; digestion; response to stress

Research Articles on UCN2

Similar Products

Product Notes

The UCN2 ucn2 (Catalog #AAA2012647) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Urocortin 2 (UCN2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Urocortin 2 (UCN2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the UCN2 ucn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG S-V PVTPIPTFQL RPQNSPQTTP RPAASESPSA APTWPWAAQS. It is sometimes possible for the material contained within the vial of "Urocortin 2 (UCN2), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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