Immunohistochemistry (IHC)
(Immunochemical staining of human UPAR in human lung cancer with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human uPAR/PLAUR Polyclonal Antibody | anti-PLAUR antibody
Anti-uPAR/PLAUR Antibody, Rabbit Polyclonal
IHC-P: 1:500-1:2000
ICC/IF: 1:100-1:500
IP: 1-5uL/mg of lysate
Immunohistochemistry (IHC)
(Immunochemical staining of human UPAR in human lung cancer with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human UPAR in human lung cancer with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human UPAR in human ovarian cancer with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human UPAR in human ovarian cancer with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunofluorescence (IF)
(Immunofluorescence staining of PLAUR in HT1080 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human PLAUR polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cell membrane and cytoplasm.)
Immunofluorescence (IF)
(Immunofluorescence staining of PLAUR in HT1080 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human PLAUR polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cell membrane and cytoplasm.)
Immunoprecipitation (IP)
(UPAR was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-UPAR rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-UPAR rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa Observed band size :37 kDa)
Immunoprecipitation (IP)
(UPAR was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-UPAR rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-UPAR rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa Observed band size :37 kDa)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol (GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. [provided by RefSeq, Jul 2008]
Uniprot Description
uPAR: Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. Monomer (Probable). Interacts with MRC2. Interacts (via the UPAR/Ly6 domains) with SRPX2. Expressed in neurons of the rolandic area of the brain. Expressed in the brain. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Membrane protein, GPI anchor; Receptor, misc.
Chromosomal Location of Human Ortholog: 19q13
Cellular Component: endoplasmic reticulum membrane; extrinsic to membrane; focal adhesion; integral to plasma membrane; endoplasmic reticulum lumen; integral to membrane; plasma membrane
Molecular Function: protein domain specific binding; protein binding; U-plasminogen activator receptor activity; enzyme binding; receptor activity; receptor binding
Biological Process: fibrinolysis; cellular protein metabolic process; attachment of GPI anchor to protein; regulation of proteolysis; C-terminal protein lipidation; chemotaxis; post-translational protein modification; cell motility; blood coagulation; signal transduction
Research Articles on PLAUR
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Product Notes
The PLAUR plaur (Catalog #AAA8104355) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-uPAR/PLAUR Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's uPAR/PLAUR can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). ELISA: 1:5000-1:10,000 IHC-P: 1:500-1:2000 ICC/IF: 1:100-1:500 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the PLAUR plaur for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "uPAR/PLAUR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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