Rabbit anti-Mouse UCHL3  Monoclonal Antibody | anti-UCHL3  antibody

Rabbit anti Mouse UCHL3  Monoclonal Antibody

Cat. Num. AAA2544643

• Gene Names: UCHL3; UCH-L3
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: UCHL3 ; Monoclonal Antibody; Rabbit anti Mouse UCHL3 Monoclonal Antibody; anti-UCHL3 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 194

• Applicable Applications for anti-UCHL3 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Mouse UCHL3. The detection limit for Mouse UCHL3 is approximately 0.0049 ng/well.
• Immunogen: Recombinant Mouse UCHL3 protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-UCHL3  antibody

Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase which belongs to the peptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 401709950
• NCBI GeneID: 7347
• NCBI GenBank Nucleotide #: NM_001270952.1
• Molecular Weight: 26,183 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform 1
• NCBI Official Synonym Full Names: ubiquitin C-terminal hydrolase L3
• NCBI Official Symbol: UCHL3
• NCBI Official Synonym Symbols: UCH-L3
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L3
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L3
• UniProt Gene Name: UCHL3
• UniProt Synonym Gene Names: UCH-L3
• UniProt Entry Name: UCHL3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]

Uniprot Description

Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked Ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders.