Rabbit anti-Human UCHL3 Polyclonal Antibody | anti-UCHL3 antibody

Anti-UCHL3 Antibody, Rabbit Polyclonal

Cat. Num. AAA8105538

• Gene Names: UCHL3; UCH-L3
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: UCHL3; Polyclonal Antibody; Anti-UCHL3 Antibody; Rabbit Polyclonal; UCHL3/UCH-L3 Antibody; Rabbit PAb; Antigen Affinity Purified; ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase); Anti-UCH-L3 Antibody; anti-UCHL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human UCHL3/UCH-L3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-UCHL3 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human UCHL3 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human UCHL3 (rh UCHL3). UCHL3 specific IgG was purified by human UCHL3 affinity chromatography .
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(UCHL3 was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate2 uL anti-UCHL3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-UCHL3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 25 kDaObserved band size: 25 kDa)

Western Blot (WB)

(Anti-UCHL3 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:18 kDaObserved band size:14 kDa)

Related Product Information for anti-UCHL3 antibody

Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase that belongs to the peptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer.

References

Wood, M.A. et al., 2005, Hippocampus 15 (5):610-21. Kwon, J. et al., 2006, Exp Anim 55 (1):35-43. Setsuie, R. et al., 2009, Neurochem Int 54 (5-6):314-21. Setsuie, R. et al., 2010, Neurochem Int 56 (8):911-8.

NCBI and Uniprot Product Information

• NCBI GI #: 5174741
• NCBI GeneID: 7347
• NCBI Accession #: NP_005993.1
• NCBI GenBank Nucleotide #: NM_006002.4
• UniProt Accession #: P15374
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform 2
• NCBI Official Synonym Full Names: ubiquitin C-terminal hydrolase L3
• NCBI Official Symbol: UCHL3
• NCBI Official Synonym Symbols: UCH-L3
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L3
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L3
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: UCHL3
• UniProt Synonym Gene Names: UCH-L3
• UniProt Entry Name: UCHL3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]

Uniprot Description

UCHL3: Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin- signaling and insulin-induced adipogenesis. Required for stress- response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders. Preferentially binds diubiquitin; the interaction does not hydrolyze diubiquitin but, in vitro, inhibits the hydrolyzing activity on other substrates. Highly expressed in heart, skeletal muscle, and testis. Inhibited by monoubiquitin and diubiquitin. Belongs to the peptidase C12 family.

Protein type: Protease; EC 3.4.19.12; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 13q22.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: peptidase activity; ubiquitin binding; protein binding; ubiquitin-specific protease activity

Biological Process: ubiquitin-dependent protein catabolic process; protein catabolic process