Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with CD19 antibody (FITC) and 0.125 ug of Rat IgG2b kappa Isotype Control (left) or 0.125 ug of PIR-A/B antibody (right). Total viable cells were used for analysis.)
Rat PIR-A/B Monoclonal Antibody | anti-PIR-A/B antibody
PIR-A/B antibody (allophycocyanin)
Gene Names
Pira1; Pir; 6M21; Ly89; PIR-A1
Applications
Flow Cytometry, Functional Assay
Synonyms
PIR-A/B; Monoclonal Antibody; PIR-A/B antibody (allophycocyanin); Monoclonal PIR-A/B; Anti-PIR-A/B; PIR-A/B (APC); PIR-A; PIR-B; PIR A; PIRAB; PIR-AB; PIR AB; PirB; PIR B; Anti PIR-A/B; PirA; anti-PIR-A/B antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
10-1-PIR
Specificity
Mouse
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
200 ug/mL (varies by lot)
Sequence Length
680
Applicable Applications for anti-PIR-A/B antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: 5 ul (0.5 ug)/test
A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Biological Significance
This 10-1-PIR monoclonal antibody reacts with mouse paired Ig-like receptors of activating -A and -B. These cell surface glycoproteins, which contain six extracellular Ig-like domains with distinct transmembrane and cytoplasmic regions, are expressed together on B lymphocytes, dendritic cells, macrophages, granulocytes, platelets, and mast cells. However, expression of PIR-A/B has not been observed on T cells, NK cells, and erythrocytes. The PIR-A receptor interacts with signaling molecules containing immunoreceptor tyrosine-based activation motifs which lead to its activating function. In contrast, the PIR-B receptor associates with proteins possessing immunoreceptor tyrosine-based inhibitory motifs therefore, this receptor has been shown to have an inhibitory function. PIRs bind MHC class I to modulate cell signaling and homeostasis of the immune system. Moreover, PIR-B knockout mice have been shown to exhibit susceptibility to Salmonella infection.Crossblocking studies indicate that 10-1-PIR recognizes a different epitope from 6C1.
Tag/Conjugate
Allophycocyanin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with CD19 antibody (FITC) and 0.125 ug of Rat IgG2b kappa Isotype Control (left) or 0.125 ug of PIR-A/B antibody (right). Total viable cells were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with CD19 antibody (FITC) and 0.125 ug of Rat IgG2b kappa Isotype Control (left) or 0.125 ug of PIR-A/B antibody (right). Total viable cells were used for analysis.)
Related Product Information for anti-PIR-A/B antibody
Rat monoclonal PIR-A/B antibody (allophycocyanin)
Product Categories/Family for anti-PIR-A/B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
paired-Ig-like receptor A1
NCBI Official Synonym Full Names
paired-Ig-like receptor A1
NCBI Official Symbol
Pira1
NCBI Official Synonym Symbols
Pir; 6M21; Ly89; PIR-A1
NCBI Protein Information
paired-Ig-like receptor A1
Research Articles on PIR-A/B
Similar Products
Product Notes
The PIR-A/B (Catalog #AAA833866) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PIR-A/B can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: 5 ul (0.5 ug)/test A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Researchers should empirically determine the suitability of the PIR-A/B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIR-A/B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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