UCHL3 recombinant protein

Recombinant Rat UCHL3 Protein (His tag)

Cat. Num. AAA2547471

• Gene Names: Uchl3; RGD1561196
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: UCHL3; Recombinant Rat UCHL3 Protein (His tag); rCG_37146; RGD1561196; UCHL3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 230

• Application Notes: The recombinant rat UCHL3 comprises 240 amino acids and predicts a molecular mass of 27.5 kDa. The apparent molecular mass of the rat UCHL3 is approximately 28 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for UCHL3 recombinant protein

Background: Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase which belongs to the peptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotype s indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer.

Description: A DNA sequence encoding the rat UCHL3 (Q91Y78) (Glu 2-Ala 230) was fused with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 158749588
• NCBI GeneID: 498560
• NCBI Accession #: NP_001103635.1
• NCBI GenBank Nucleotide #: NM_001110165.1
• UniProt Accession #: Q91Y78
• Molecular Weight: 26,124 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L3
• NCBI Official Synonym Full Names: ubiquitin C-terminal hydrolase L3
• NCBI Official Symbol: Uchl3
• NCBI Official Synonym Symbols: RGD1561196
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L3
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L3
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: Uchl3
• UniProt Synonym Gene Names: UCH-L3

NCBI Description

mouse homolog is an enzyme involved in proteolytic processing of polymeric ubiquitin [RGD, Feb 2006]

Uniprot Description

UCHL3: Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin- signaling and insulin-induced adipogenesis. Required for stress- response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders. Preferentially binds diubiquitin; the interaction does not hydrolyze diubiquitin but, in vitro, inhibits the hydrolyzing activity on other substrates. Highly expressed in heart, skeletal muscle, and testis. Inhibited by monoubiquitin and diubiquitin. Belongs to the peptidase C12 family.

Protein type: EC 3.4.19.12; Protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 15q21

Cellular Component: cytoplasm; nucleus

Molecular Function: peptidase activity; ubiquitin binding; ubiquitin-specific protease activity

Biological Process: adult walking behavior; cellular response to insulin stimulus; eating behavior; positive regulation of fat cell differentiation; protein catabolic process; protein deubiquitination; retina development in camera-type eye