Mouse UBE2D2 / UBCH5B Monoclonal Antibody | anti-UBE2D2 antibody

Mouse Monoclonal [clone 4A1] (IgG2a,k) to Human UBE2D2 / UBCH5B

Cat. Num. AAA246442

• Gene Names: UBE2D2; UBC4; PUBC1; UBCH4; UBC4/5; UBCH5B; E2(17)KB2
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: UBE2D2 / UBCH5B; Monoclonal Antibody; Mouse Monoclonal [clone 4A1] (IgG2a; k) to Human UBE2D2 / UBCH5B; Anti-UBE2D2 / UBCH5B Antibody (clone 4A1) IHC-plus; UBE2D2; E2(17)KB2; PUBC1; UBCH4; UBCH5B; UBC4/5; UBC5B; Ubiquitin carrier protein D2; UBC4; Ubiquitin-protein ligase D2; Human UBE2D2; anti-UBE2D2 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 4A1
• Specificity: Human UBE2D2
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 0.4 mg/ml (varies by lot)
• Sequence Length: 147

• Applicable Applications for anti-UBE2D2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell line lysates and the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: UBE2D2 (NP_003330, 1 a.a. ~ 95 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: UBE2D2 / UBCH5B antibody was raised against uBE2D2 (NP_003330, 1 a.a. ~ 95 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-UBE2D2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(UBE2D2 monoclonal antibody clone 4A1 Western blot of UBE2D2 expression in NIH/3T3.)

Western Blot (WB)

(UBE2D2 monoclonal antibody clone 4A1. Western blot of UBE2D2 expression in PC-12.)

ELISA (EIA)

(Detection limit for recombinant GST tagged UBE2D2 is approximately 3 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 4507775
• NCBI GeneID: 7322
• NCBI Accession #: NP_003330.1
• NCBI GenBank Nucleotide #: NM_003339.2
• UniProt Accession #: P62837; P51669; Q3MN78; Q96RP6; D3DQC9
• Molecular Weight: 13,635 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 D2 isoform 1
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2D 2
• NCBI Official Symbol: UBE2D2
• NCBI Official Synonym Symbols: UBC4; PUBC1; UBCH4; UBC4/5; UBCH5B; E2(17)KB2
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 D2; ubiquitin-protein ligase D2; ubiquitin carrier protein D2; ubiquitin-conjugating enzyme E2-17 kDa 2; p53-regulated ubiquitin-conjugating enzyme 1; ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5)
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 D2
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2D2
• UniProt Synonym Gene Names: PUBC1; UBC4; UBC5B; UBCH4; UBCH5B
• UniProt Entry Name: UB2D2_HUMAN

NCBI Description

Regulated degradation of misfolded, damaged or short-lived proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome system (UPS). An integral part of the UPS system is the ubiquitination of target proteins and covalent linkage of Ub-containing proteins to form polymeric chains, marking them as targets for 26S proteasome-mediated degradation. Ubiquitination of proteins is mediated by a cascade of enzymes which includes E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligases) enzymes. This gene encodes a member of the E2 enzyme family. Substrates of this enzyme include the tumor suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]

Uniprot Description

UBE2D2: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP- induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: EC 6.3.2.19; Ligase; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: nucleoplasm; protein complex; cytosol; ubiquitin ligase complex

Molecular Function: protein binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; protein polyubiquitination; MyD88-independent toll-like receptor signaling pathway; toll-like receptor signaling pathway; protein ubiquitination; protein modification process; innate immune response; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway