Rabbit UBE2D2 Polyclonal Antibody | anti-UBE2D2 antibody

Anti-UBE2D2 Antibody

Cat. Num. AAA820197

• Gene Names: UBE2D2; UBC4; PUBC1; UBCH4; UBC4/5; UBCH5B; E2(17)KB2
• Reactivity: Human, Mouse, Rat, Bovine, Pig
• Applications: Western Blot, Immunoprecipitation
• Purity: The antibody was purified by immunogen affinity chromatography.
• Synonyms: UBE2D2; Polyclonal Antibody; Anti-UBE2D2 Antibody; PUBC1; UBC4; UBC5B; UBCH4; UBCH5B; Ubiquitin-conjugating enzyme E2 D2; Ubiquitin carrier protein D2; Ubiquitin-conjugating enzyme E2(17)KB 2; Ubiquitin-conjugating enzyme E2-17 kDa 2; Ubiquitin-protein ligase D2; p53-regulated ubiquitin-conjugating enzyme 1; anti-UBE2D2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine, Pig
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of UBE2D2 protein.
• Purity/Purification: The antibody was purified by immunogen affinity chromatography.
• Form/Format: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Sequence Length: 147

• Applicable Applications for anti-UBE2D2 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB (1/500 - 1/1000), IP (1/10 - 1/100)
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human UBE2D2. The exact sequence is proprietary.
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of UBE2D2 expression in MCF7 (A), HepG2 (B), NIH3T3 (C), H9C2 (D) whole cell lysates.)

Related Product Information for anti-UBE2D2 antibody

Rabbit polyclonal antibody to UBE2D2

NCBI and Uniprot Product Information

• NCBI GI #: 4507775
• NCBI GeneID: 7322
• NCBI Accession #: NP_003330.1
• NCBI GenBank Nucleotide #: NM_003339.2
• UniProt Accession #: P62837; P51669; Q3MN78; Q96RP6; D3DQC9
• Molecular Weight: 13,635 Da
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 D2 isoform 1
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2D 2
• NCBI Official Symbol: UBE2D2
• NCBI Official Synonym Symbols: UBC4; PUBC1; UBCH4; UBC4/5; UBCH5B; E2(17)KB2
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 D2; ubiquitin-protein ligase D2; ubiquitin carrier protein D2; ubiquitin-conjugating enzyme E2-17 kDa 2; p53-regulated ubiquitin-conjugating enzyme 1; ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5)
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 D2
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2D2
• UniProt Synonym Gene Names: PUBC1; UBC4; UBC5B; UBCH4; UBCH5B
• UniProt Entry Name: UB2D2_HUMAN

NCBI Description

Regulated degradation of misfolded, damaged or short-lived proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome system (UPS). An integral part of the UPS system is the ubiquitination of target proteins and covalent linkage of Ub-containing proteins to form polymeric chains, marking them as targets for 26S proteasome-mediated degradation. Ubiquitination of proteins is mediated by a cascade of enzymes which includes E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligases) enzymes. This gene encodes a member of the E2 enzyme family. Substrates of this enzyme include the tumor suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]

Uniprot Description

UBE2D2: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP- induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: nucleoplasm; protein complex; cytosol; ubiquitin ligase complex

Molecular Function: protein binding; ubiquitin-protein ligase activity; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; protein polyubiquitination; MyD88-independent toll-like receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; protein modification process; protein ubiquitination; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway