Mouse anti-Human, Mouse UBC9 Monoclonal Antibody | anti-UBC9 antibody

UBC9 (SUMO-conjugating Enzyme UBC9, SUMO-protein Ligase, Ubiquitin-conjugating Enzyme E2 I, Ubiquitin-protein Ligase I, Ubiquitin Carrier Protein I, Ubiquitin Carrier Protein 9, p18, UBE2I, UBC9, UBCE9)

Cat. Num. AAA609974

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: UBC9; Monoclonal Antibody; UBC9 (SUMO-conjugating Enzyme UBC9; SUMO-protein Ligase; Ubiquitin-conjugating Enzyme E2 I; Ubiquitin-protein Ligase I; Ubiquitin Carrier Protein I; Ubiquitin Carrier Protein 9; p18; UBE2I; UBCE9); Anti -UBC9 (SUMO-conjugating Enzyme UBC9; anti-UBC9 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10i82
• Specificity: Recognizes human UBC9. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-UBC9 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500
• Immunogen: Purified GST-UBC9 fusion protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-UBC9 Antibody in Ramos cell line lysates (35ug/lane). UBC9(arrow) was detected using the purified Mab.)

Western Blot (WB)

(Western blot analysis of anti-UBC9 Antibody in mouse spleen tissue lysates (35ug/lane). UBC9(arrow) was detected using the purified Mab.)

Related Product Information for anti-UBC9 antibody

Human Ubquitin Conjugating Enzyme 9 (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumoylation, and may play a role in substrate recognition. Ubc9 can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, IkBa, and PML without the requirement of an E3 ligase. Ubc9 is essential for nuclear architecture and chromosome segregation.

Product Categories/Family for anti-UBC9 antibody

Antibodies; Abs to Ubiquitin Ligases

NCBI and Uniprot Product Information

• NCBI GI #: 1431070
• UniProt Accession #: P50623; D6VRT3
• Molecular Weight: 17,911 Da
• NCBI Official Full Name: UBC9
• UniProt Protein Name: SUMO-conjugating enzyme UBC9
• UniProt Gene Name: UBC9
• UniProt Entry Name: UBC9_YEAST

Uniprot Description

Function: E2 ubiquitin-like--protein ligase mediating SUMO/Smt3 attachment to septins and PCNA. Seems to be involved in degradation of S- (CLB5) and M-phase cyclins (CLB2). Ref.4 Ref.5

Catalytic activity: ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine.

Pathway: Protein modification; protein sumoylation.

Subunit structure: Interacts with SIZ1. Ref.5 Ref.6

Subcellular location: Nucleus Ref.7.

Miscellaneous: Present with 2600 molecules/cell in log phase SD medium. Ref.8

Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.