Mouse anti-Human, Mouse UBC9 Monoclonal Antibody | anti-UBC9 antibody

UBC9 (SUMO-conjugating Enzyme UBC9, SUMO-protein Ligase, Ubiquitin-conjugating Enzyme E2 I, Ubiquitin-protein Ligase I, Ubiquitin Carrier Protein I, Ubiquitin Carrier Protein 9, p18, UBE2I, UBC9, UBCE9) (APC)

Cat. Num. AAA6120936

• Gene Names: UBE2I; P18; UBC9; C358B7.1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: UBC9; Monoclonal Antibody; UBC9 (SUMO-conjugating Enzyme UBC9; SUMO-protein Ligase; Ubiquitin-conjugating Enzyme E2 I; Ubiquitin-protein Ligase I; Ubiquitin Carrier Protein I; Ubiquitin Carrier Protein 9; p18; UBE2I; UBCE9) (APC); anti-UBC9 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10i82
• Specificity: Recognizes human UBC9. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-UBC9 antibody: FLISA, Western Blot (WB)
• Application Notes: FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Purified GST-UBC9 fusion protein.
• Conjugate: APC
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-UBC9 Antibody in Ramos cell line lysates (35ug/lane). UBC9 (arrow) was detected using the purified Mab.)

Western Blot (WB)

(Western blot analysis of anti-UBC9 Antibody in mouse spleen tissue lysates (35ug/lane). UBC9 (arrow) was detected using the purified Mab.)

Product Categories/Family for anti-UBC9 antibody

Antibodies; Ubiquitin Ligases

References

1. Weldon, R.A. Jr., et al., Virology 314(1):62-73 (2003). 2.Lin, X., et al., J. Biol. Chem. 278(33):31043-31048 (2003). 3.Netzer, C., et al., Biochem. Biophys. Res. Commun. 296(4):870-876 (2002). 4.Sheng, W., et al., Protein Sci. 11(6):1482-1491 (2002). 5.Lin, D., et al., J. Biol. Chem. 277(24):21740-21748 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 4507785
• NCBI GeneID: 7329
• NCBI Accession #: NP_003336
• NCBI GenBank Nucleotide #: NM_003345.4
• UniProt Accession #: P63279; P50550; Q15698; Q59GX1; Q86VB3; D3DU69
• Molecular Weight: 18 kDa (158 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: SUMO-conjugating enzyme UBC9
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 I
• NCBI Official Symbol: UBE2I
• NCBI Official Synonym Symbols: P18; UBC9; C358B7.1
• NCBI Protein Information: SUMO-conjugating enzyme UBC9
• UniProt Protein Name: SUMO-conjugating enzyme UBC9
• UniProt Gene Name: UBE2I
• UniProt Synonym Gene Names: UBC9; UBCE9
• UniProt Entry Name: UBC9_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]