Rabbit anti-Human UBC9 Polyclonal Antibody | anti-UBC9 antibody

UBC9, NT (SUMO-conjugating Enzyme UBC9, SUMO-protein Ligase, Ubiquitin-conjugating Enzyme E2 I, Ubiquitin-protein Ligase I, Ubiquitin Carrier Protein I, Ubiquitin Carrier Protein 9, p18, UBE2I, UBC9, UBCE9)

Cat. Num. AAA626003

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by ammonium sulfate precipitation.
• Synonyms: UBC9; Polyclonal Antibody; NT (SUMO-conjugating Enzyme UBC9; SUMO-protein Ligase; Ubiquitin-conjugating Enzyme E2 I; Ubiquitin-protein Ligase I; Ubiquitin Carrier Protein I; Ubiquitin Carrier Protein 9; p18; UBE2I; UBCE9); Anti -UBC9; anti-UBC9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UBC9
• Purity/Purification: Affinity Purified; Purified by ammonium sulfate precipitation.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.
• Concentration: 1.64mg/ml (varies by lot)

• Applicable Applications for anti-UBC9 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Western Blot: 1:1000; Immunohistochemistry: 1:50-1:100; Optimal dilutions to be determined by the researcher.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human UBC19, UniProt Accession #P63279.
• Grade: Purified
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of A2058 cell line lysates (35ug/lane) using MBS626003. UBE2I (arrow) was detected using the U0880-03.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue using MBS626003, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot analysis of 293 cell lysates (2ug/lane) using MBS626003 on either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2I gene.)

Related Product Information for anti-UBC9 antibody

UBE2I (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumoylation, and may play a role in substrate recognition. UBE2I can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, IκBα, and PML without the requirement of an E3 ligase. UBE2I is essential for nuclear architecture and chromosome segregation.

Product Categories/Family for anti-UBC9 antibody

Antibodies; Abs to Ubiquitin Ligases

NCBI and Uniprot Product Information

• UniProt Accession #: P63279
• Molecular Weight: 17,911 Da
• NCBI Official Full Name: UBC9
• UniProt Protein Name: SUMO-conjugating enzyme UBC9
• UniProt Gene Name: UBC9
• UniProt Entry Name: UBC9_YEAST

Uniprot Description

Function: E2 ubiquitin-like--protein ligase mediating SUMO/Smt3 attachment to septins and PCNA. Seems to be involved in degradation of S- (CLB5) and M-phase cyclins (CLB2). Ref.4 Ref.5

Catalytic activity: ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine.

Pathway: Protein modification; protein sumoylation.

Subunit structure: Interacts with SIZ1. Ref.5 Ref.6

Subcellular location: Nucleus Ref.7.

Miscellaneous: Present with 2600 molecules/cell in log phase SD medium. Ref.8

Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.