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Mouse TUBULIN BETA Monoclonal Antibody

MOUSE ANTI PIG TUBULIN BETA (N-TERMINAL)

Reactivity
Human, Mouse, Plants, Rat
Applications
Immunohistochemistry, ELISA, Western Blot
Synonyms
TUBULIN BETA; Monoclonal Antibody; MOUSE ANTI PIG TUBULIN BETA (N-TERMINAL); anti-TUBULIN BETA antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Plants, Rat
Clonality
Monoclonal
Isotype
IgM
Clone Number
TU-13
Specificity
This item recognises an epitope within the N-terminal region (NT) of tubulin beta, a 50kDa globular protein and major constituent of microtubules within the cytoskeleton, involved in meiosis, mitosis, intracellular transport and maintaining cell shape.The evolutionarily conserved heterodimer tubulin, made up of a tubulin alpha and beta chain, binds two molecules of GTP, one at a non-exchangeable site on the alpha chain and one at an exchangeable site on the beta chain. Only the beta chain possesses GTPase activity however, hydrolysing GTP to GDP, and influencing the stability of the dimer within the microtubule.Clone TU-13 is reported to partially inhibit microtubule assembly in vitro, see Draber, P. et al. for details. This item is reported as suitable for use in immunocytochemistry.
Form/Format
Purified
Purified IgM - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
445
Applicable Applications for anti-TUBULIN BETA antibody
Immunohistology Frozen, ELISA (EIA), Western Blot (WB)
Application Notes
Western Blot: This item detects a band of approximately 50kDa in human RAJI and mouse EL4 cell lysates.
Western Blotting: Maximum Dilution: 1.0ug/ml
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Immunogen
Tubulin beta from porcine brain.
Buffer Solution
Target Species
Pig
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Related Product Information for anti-TUBULIN BETA antibody
MBS214464 recognises an epitope within the N-terminal region (NT) of tubulin beta, a 50kDa globular protein and major constituent of microtubules within the cytoskeleton, involved in meiosis, mitosis, intracellular transport and maintaining cell shape. The evolutionarily conserved heterodimer tubulin, made up of a tubulin alpha and beta chain, binds two molecules of GTP, one at a non-exchangeable site on the alpha chain and one at an exchangeable site on the beta chain. Only the beta chain possesses GTPase activity however, hydrolysing GTP to GDP, and influencing the stability of the dimer within the microtubule. Clone TU-13 is reported to partially inhibit microtubule assembly in vitro, see Draber, P. et al. for details. MBS214464 is reported as suitable for use in immunocytochemistry.

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
49,861 Da
NCBI Official Full Name
Tubulin beta chain
UniProt Protein Name
Tubulin beta chain
Protein Family
UniProt Entry Name
TBB_PIG

Uniprot Description

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

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Product Notes

The TUBULIN BETA (Catalog #AAA214464) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI PIG TUBULIN BETA (N-TERMINAL) reacts with Human, Mouse, Plants, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TUBULIN BETA can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, ELISA (EIA), Western Blot (WB). Western Blot: This item detects a band of approximately 50kDa in human RAJI and mouse EL4 cell lysates. Western Blotting: Maximum Dilution: 1.0ug/ml. Researchers should empirically determine the suitability of the TUBULIN BETA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TUBULIN BETA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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