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Western Blot (WB) (Western blot detection of Tubulin beta in COS7, LNCap, MCF7 and C2C12 cell lysates and using Tubulin beta mouse mAb (1:5000 diluted).Predicted band size: 55KDa.Observed band size: 55KDa.)

beta-Tubulin Monoclonal Antibody | anti-TUBB antibody

Anti-beta-Tubulin Mouse mAb

Gene Names
TUBB; M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56
Reactivity
Human, Mouse, Monkey, Goat, Hamster, Rat, Chlamydomonas
Applications
Western Blot, Immunofluorescence
Purity
Affinity Purified
Synonyms
beta-Tubulin; Monoclonal Antibody; Anti-beta-Tubulin Mouse mAb; beta 4 tubulin; Beta 5 tubulin; beta Ib tubulin; M40; TUBB1; TUBB5; tubulin beta 1 chain; Tubulin beta 4 chain; tubulin beta 5 chain; Tubulin beta; Tubulin beta chain; tubulin beta polypeptide; anti-TUBB antibody
Ordering
For Research Use Only!
Host
Mouse
Source: Human
Reactivity
Human, Mouse, Monkey, Goat, Hamster, Rat, Chlamydomonas
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1E1-E8-H4
Purity/Purification
Affinity Purified
Form/Format
Purified mouse monoclonal in PBS (pH 7.4) containing with 0.02% sodium azide, 0.1mg/mlBSA and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
464
Applicable Applications for anti-TUBB antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:5000
ICC: 1:500
Immunogen
Purified recombinant human Tubulin beta protein fragments expressed in E Coli.
Sensitivity
This antibody detects endogenous levels of Tubulin beta and does not cross-react with related proteins.
Sequence Similarities
Belongs to the tubulin family.
Subunit Structure
Dimer of alpha and beta chains.May interact with RNABP10 By similarity.Interacts with PIFO.Interacts with MX1.
Subcellular Location
Cytoplasm; cytoskeleton.

Western Blot (WB)

(Western blot detection of Tubulin beta in COS7, LNCap, MCF7 and C2C12 cell lysates and using Tubulin beta mouse mAb (1:5000 diluted).Predicted band size: 55KDa.Observed band size: 55KDa.)

Western Blot (WB) (Western blot detection of Tubulin beta in COS7, LNCap, MCF7 and C2C12 cell lysates and using Tubulin beta mouse mAb (1:5000 diluted).Predicted band size: 55KDa.Observed band size: 55KDa.)

Immunocytochemistry (ICC)

(Immunocytochemistry staining of HeLa cells fixed with 4% paraformaldehyde and using anti-Tubulin beta mouse mAb (dilution 1:500).)

Immunocytochemistry (ICC) (Immunocytochemistry staining of HeLa cells fixed with 4% paraformaldehyde and using anti-Tubulin beta mouse mAb (dilution 1:500).)

Testing Data

(Tubulin beta antibody's stability test under 37 degree C 50 hours compare to 4 degree C 50 hours.)

Testing Data (Tubulin beta antibody's stability test under 37 degree C 50 hours compare to 4 degree C 50 hours.)

Western Blot (WB)

(Western blot detection of Tubulin beta in goat intestine, goat skeletal muscle, goat spleen and goat lung cell lysates using Tubulin beta mouse mAb (1:5000-1:10000 diluted).Predicted band size: 55KDa.Observed band size: 55KDa.)

Western Blot (WB) (Western blot detection of Tubulin beta in goat intestine, goat skeletal muscle, goat spleen and goat lung cell lysates using Tubulin beta mouse mAb (1:5000-1:10000 diluted).Predicted band size: 55KDa.Observed band size: 55KDa.)

Western Blot (WB)

(Western blot detection of Tubulin beta in Goat skeletal muscle, CHO-k1, COS7, C6, 3T3 and K562 cell lysates using Tubulin beta mouse mAb (1:5000 diluted).Predicted band size:55KDa.Observed band size:55KDa.Exposure time:10sec.)

Western Blot (WB) (Western blot detection of Tubulin beta in Goat skeletal muscle, CHO-k1, COS7, C6, 3T3 and K562 cell lysates using Tubulin beta mouse mAb (1:5000 diluted).Predicted band size:55KDa.Observed band size:55KDa.Exposure time:10sec.)

Western Blot (WB)

(Western blot detection of beta Tubulin in Chlamydomonas cell lysates using beta Tubulin mouse mAb (1:1000 diluted).Predicted band size:55KDa.Observed band size:55KDa.)

Western Blot (WB) (Western blot detection of beta Tubulin in Chlamydomonas cell lysates using beta Tubulin mouse mAb (1:1000 diluted).Predicted band size:55KDa.Observed band size:55KDa.)
Related Product Information for anti-TUBB antibody
UniPort Summary Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
55
NCBI Official Full Name
tubulin beta chain isoform a
NCBI Official Synonym Full Names
tubulin beta class I
NCBI Official Symbol
TUBB
NCBI Official Synonym Symbols
M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56
NCBI Protein Information
tubulin beta chain
UniProt Protein Name
Tubulin beta chain
Protein Family
UniProt Gene Name
TUBB
UniProt Synonym Gene Names
TUBB5

NCBI Description

This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]

Uniprot Description

TUBB: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. May interact with RNABP10. Interacts with PIFO/C1orf88. Ubiquitously expressed with highest levels in spleen, thymus and immature brain. Belongs to the tubulin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: cytoskeleton; extracellular matrix; extracellular region; microtubule; nuclear envelope lumen; nucleus

Molecular Function: MHC class I protein binding; protein binding; structural molecule activity; ubiquitin protein ligase binding

Biological Process: cell division; cell motility; cellular process; cytoskeleton-dependent intracellular transport; G2/M transition of mitotic cell cycle; microtubule-based process

Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 6; Skin Creases, Congenital Symmetric Circumferential, 1

Research Articles on TUBB

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Product Notes

The TUBB tubb (Catalog #AAA475115) is an Antibody produced from Mouse Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-beta-Tubulin Mouse mAb reacts with Human, Mouse, Monkey, Goat, Hamster, Rat, Chlamydomonas and may cross-react with other species as described in the data sheet. AAA Biotech's beta-Tubulin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:5000 ICC: 1:500. Researchers should empirically determine the suitability of the TUBB tubb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Tubulin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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