Western Blot (WB)
(Cell lysates (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TUBB2b (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Mouse anti-Human, Mouse TUBB2b Monoclonal Antibody | anti-TUBB2b antibody
TUBB2b antibody
Gene Names
TUBB2B; CDCBM7; PMGYSA; bA506K6.1
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
Purity
By protein-G affinity chromatography
Synonyms
TUBB2b; Monoclonal Antibody; TUBB2b antibody; bA506K6.1; DKFZp566F223; FLJ98847; MGC8685; Tubulin beta-2B chain; tubulin beta 2B; anti-TUBB2b antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT5B3
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
445
Applicable Applications for anti-TUBB2b antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
The antibody has been tested by ELISA, Western blot, and Immunofluorescence analyses to assure specificity and reactivity. Since applications vary, however, each investigation should be titrated by a reagent to obtain optimal results.
Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500.
Recommended starting dilution is 1:250
Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500.
Recommended starting dilution is 1:250
Antigen Species
Human
Immunogen
Recombinant human TUBB2b (1-445aa) purified from E. coli
Clone Note
Anti-human TUBB2b mAb, clone AT5B3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TUBB2b protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(Cell lysates (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TUBB2b (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Western Blot (WB)
(Cell lysates (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TUBB2b (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Immunofluorescence (IF)
(Immunofluorescence of human SW480 cells stained with monoclonal anti-human TUBB2b antibody (1:250) with Texas Red (Red). Arrow head: Mitotic cell, Arrow: MTOC (microtubule organizing center).)
Immunofluorescence (IF)
(Immunofluorescence of human SW480 cells stained with monoclonal anti-human TUBB2b antibody (1:250) with Texas Red (Red). Arrow head: Mitotic cell, Arrow: MTOC (microtubule organizing center).)
Related Product Information for anti-TUBB2b antibody
The protein encoded by this gene is a beta isoform of tubulin, which binds GTP and is a major component of microtubules. Multiple beta Tubulin isoforms have been characterized and are expressed in mammalian tissues. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB2b is mainly expressed in developing neurons with a dominant expression during the critical steps of corticogenesis. Also, TUBB2B is a new critical gene involved in neuronal migration. It is present in the nuclei and nucleoplasm, Defects in this gene are a cause of asymmetric polymicrogyria.
Product Categories/Family for anti-TUBB2b antibody
References
Xavier Hubert Jaglin, et al. (2009) Nat Genet, 41 (6):746-52.; Bouwmeester T, et al. (2004) Nat Cell Bio, 6 (2):97-105.; Modig, C, et al. (1999) Cell Motil Cytoskeleton, 42 (4): 315-330.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,953 Da
NCBI Official Full Name
tubulin beta-2B chain
NCBI Official Synonym Full Names
tubulin beta 2B class IIb
NCBI Official Symbol
TUBB2B
NCBI Official Synonym Symbols
CDCBM7; PMGYSA; bA506K6.1
NCBI Protein Information
tubulin beta-2B chain
UniProt Protein Name
Tubulin beta-2B chain
Protein Family
UniProt Gene Name
TUBB2B
NCBI Description
The protein encoded by this gene is a beta isoform of tubulin, which binds GTP and is a major component of microtubules. This gene is highly similar to TUBB2A and TUBB2C. Defects in this gene are a cause of asymmetric polymicrogyria. [provided by RefSeq, Mar 2010]
Uniprot Description
Tubulin is the major constituent of microtubules (PubMed:23001566, PubMed:28013290, PubMed:26732629). It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (). Plays a critical role in proper axon guidance in both central and peripheral axon tracts (PubMed:23001566). Implicated in neuronal migration (PubMed:19465910).
Research Articles on TUBB2b
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Product Notes
The TUBB2b tubb2b (Catalog #AAA200174) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TUBB2b antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TUBB2b can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC). The antibody has been tested by ELISA, Western blot, and Immunofluorescence analyses to assure specificity and reactivity. Since applications vary, however, each investigation should be titrated by a reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500. Recommended starting dilution is 1:250. Researchers should empirically determine the suitability of the TUBB2b tubb2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TUBB2b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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