Mouse anti-Human TUBA4A / TUBA1 Monoclonal Antibody | anti-TUBA4A antibody

Mouse Monoclonal [clone 2E11] (IgG2b,k) to Human TUBA4A / TUBA1

Cat. Num. AAA245760

• Gene Names: TUBA4A; TUBA1; H2-ALPHA
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: TUBA4A / TUBA1; Monoclonal Antibody; Mouse Monoclonal [clone 2E11] (IgG2b; k) to Human TUBA4A / TUBA1; Anti-TUBA4A / TUBA1 Antibody (clone 2E11) IHC-plus; TUBA4A; Alpha-tubulin 1; TUBA1; Testis-specific alpha-tubulin; Tubulin; alpha 4a; H2-ALPHA; Tubulin alpha-1 chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin H2-alpha; alpha 1; Human TUBA4A; anti-TUBA4A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 2E11
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 448

• Applicable Applications for anti-TUBA4A antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA), RNA interference
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), RNAi, WB; Usage: Western Blot (Cell lysate) - positive control HeLa. Western Blot (Cell lysate) - positive control different Cell lines and Human Tissue. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) -...
• Target Species: Human
• Immunogen Description: TUBA1 (AAH09238, 1 a.a. ~ 449 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: TUBA4A / TUBA1 antibody was raised against tUBA1 (AAH09238, 1 a.a. ~ 449 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-TUBA4A antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-TUBA4A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-TUBA4A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to TUBA1 on HeLa cell (antibody concentration 10 ug/ml).)

Western Blot (WB)

(TUBA1 monoclonal antibody, clone 2E11. Western blot of TUBA1 expression in different Cell lines and Human Tissue.)

RNA interference

(Western blot of TUBA1 over-expressed 293 cell line, cotransfected with TUBA1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TUBA1 monoclonal antibody, clone 2E11. GAPDH (36.1 kD) used as specificity.)

Related Product Information for anti-TUBA4A antibody

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes.

NCBI and Uniprot Product Information

• NCBI GI #: 17921989
• NCBI GeneID: 7277
• NCBI Accession #: NP_005991.1
• NCBI GenBank Nucleotide #: NM_006000.2
• UniProt Accession #: P68366; P05215; A8MUB1; B3KNQ6
• Molecular Weight: 48,329 Da
• NCBI Official Full Name: tubulin alpha-4A chain isoform 1
• NCBI Official Synonym Full Names: tubulin, alpha 4a
• NCBI Official Symbol: TUBA4A
• NCBI Official Synonym Symbols: TUBA1; H2-ALPHA
• NCBI Protein Information: tubulin alpha-4A chain; tubulin H2-alpha; tubulin alpha-1 chain; tubulin, alpha 1 (testis specific)
• UniProt Protein Name: Tubulin alpha-4A chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBA4A
• UniProt Synonym Gene Names: TUBA1
• UniProt Entry Name: TBA4A_HUMAN

NCBI Description

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

TUBA4A: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: microtubule; extracellular region; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton

Biological Process: protein polymerization; platelet activation; 'de novo' posttranslational protein folding; cellular protein metabolic process; platelet degranulation; protein folding; organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; blood coagulation; microtubule-based process

Disease: Amyotrophic Lateral Sclerosis 22 With Or Without Frontotemporal Dementia