Goat TUBA4A Polyclonal Antibody | anti-TUBA4A antibody

Tubulin Alpha4A Chain Polyclonal Antibody

Cat. Num. AAA448052

• Gene Names: TUBA4A; TUBA1; H2-ALPHA
• Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.
• Applications: Immunofluorescence, Western Blot
• Synonyms: TUBA4A; Polyclonal Antibody; Tubulin Alpha4A Chain Polyclonal Antibody; Anti-TUBA4A; TUBA1; TUBA4A antibody; anti-TUBA4A antibody

• Host: Goat
• Reactivity: Reacts against human, rat, mouse, canine and monkey proteins.
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This antibody gives a positive signal in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), canine (D17) and monkey (COS-7) whole cell lysates.
• Form/Format: Polyclonal antibody supplied as (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
• Sequence Length: 448

• Applicable Applications for anti-TUBA4A antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Western Blot: 1:250-1:2,000; Immunofluorescence: 1:50-1:200
• Gene Identifier: ENSG00000127824
• Immunogen: Purified recombinant peptide derived from within residues 400 aa to the C-terminus of human alpha tubulin produced in E. coli.
• Preparation and Storage: For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.

Reactivity Chart

Testing Data

Related Product Information for anti-TUBA4A antibody

Goat polyclonal antibody to alpha tubulin. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly.

NCBI and Uniprot Product Information

• NCBI GI #: 17921989
• NCBI GeneID: 7277
• NCBI Accession #: NP_005991.1
• NCBI GenBank Nucleotide #: NM_006000.2
• UniProt Accession #: P68366; P05215; B3KNQ6
• NCBI Official Full Name: tubulin alpha-4A chain isoform 1
• NCBI Official Synonym Full Names: tubulin, alpha 4a
• NCBI Official Symbol: TUBA4A
• NCBI Official Synonym Symbols: TUBA1; H2-ALPHA
• NCBI Protein Information: tubulin alpha-4A chain; tubulin H2-alpha; tubulin alpha-1 chain; tubulin, alpha 1 (testis specific)
• UniProt Protein Name: Tubulin alpha-4A chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBA4A
• UniProt Synonym Gene Names: TUBA1
• UniProt Entry Name: TBA4A_HUMAN

NCBI Description

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Subunit structure: Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.

Subcellular location: Cytoplasm › cytoskeleton.

Post-translational modification: Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules

Probable.Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling

By similarity.

Miscellaneous: This tubulin does not have a C-terminal tyrosine.

Sequence similarities: Belongs to the tubulin family.