Human Transcriptional activator Myb ELISA Kit | MYB elisa kit

Human Transcriptional activator Myb ELISA Kit

Cat. Num. AAA763492

• Gene Names: MYB; efg; Cmyb; c-myb; c-myb_CDS
• Reactivity: Human
• Synonyms: Transcriptional activator Myb; Human Transcriptional activator Myb ELISA Kit; MYB/Transcriptional activator Myb/Proto-oncogene c-Myb; MYB elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MYB. No significant cross-reactivity or interference between MYB and analogues was observed.
• Sequence Length: 761

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MYB elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 194328729
• NCBI GeneID: 4602
• NCBI Accession #: NP_001123645.1
• NCBI GenBank Nucleotide #: NM_001130173.1
• Molecular Weight: 37,507 Da
• NCBI Official Full Name: transcriptional activator Myb isoform 1
• NCBI Official Synonym Full Names: v-myb avian myeloblastosis viral oncogene homolog
• NCBI Official Symbol: MYB
• NCBI Official Synonym Symbols: efg; Cmyb; c-myb; c-myb_CDS
• NCBI Protein Information: transcriptional activator Myb
• UniProt Protein Name: Transcriptional activator Myb
• Protein Family: Transcriptional activator
• UniProt Gene Name: MYB
• UniProt Entry Name: MYB_HUMAN

NCBI Description

This gene encodes a protein with three HTH DNA-binding domains that functions as a transcription regulator. This protein plays an essential role in the regulation of hematopoiesis. This gene may be aberrently expressed or rearranged or undergo translocation in leukemias and lymphomas, and is considered to be an oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

Myb: Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 6q22-q23

Cellular Component: cytosol; nuclear matrix

Molecular Function: protein binding

Biological Process: B cell differentiation; blood coagulation; calcium ion transport; chromatin remodeling; embryonic gut development; G1/S transition of mitotic cell cycle; homeostasis of number of cells; in utero embryonic development; myeloid cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of histone H3-K4 methylation; positive regulation of histone H3-K9 methylation; positive regulation of T-helper cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; spleen development; stem cell division; thymus development; transcription from RNA polymerase II promoter