Rabbit anti-Human TrkA/NTRK1  Monoclonal Antibody | anti-TrkA/NTRK1  antibody

Rabbit anti Human TrkA/NTRK1  Monoclonal Antibody

Cat. Num. AAA2544162

• Gene Names: NTRK1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
• Reactivity: Human
• Applications: ELISA
• Synonyms: TrkA/NTRK1 ; Monoclonal Antibody; Rabbit anti Human TrkA/NTRK1 Monoclonal Antibody; anti-TrkA/NTRK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 796

• Applicable Applications for anti-TrkA/NTRK1 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human NTRK1. The detection limit for Human NTRK1 is 0.00245 ng/well.
• Immunogen: Recombinant Human NTRK1 protein
• Buffer: This antibody was obtained from a rabbit immunized with purified, recombinant Human NTRK1/Trk-A (rh NTRK1; NP_002520.2; Met 1-Pro 382).
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -70 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-TrkA/NTRK1  antibody

TRKA is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. It is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed. Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors. The presence of NTRK1 leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in TRKA gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. It was originally identified as an oncogene as it is commonly mutated in cancers, particularly colon and thyroid carcinomas. TRKA is required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain-derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. NTRK1 has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. It also activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway. Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis and thyroid papillary carcinoma.

NCBI and Uniprot Product Information

• NCBI GI #: 3869113
• NCBI GeneID: 4914
• Molecular Weight: 77,145 Da
• NCBI Official Full Name: TRKA
• NCBI Official Synonym Full Names: neurotrophic receptor tyrosine kinase 1
• NCBI Official Symbol: NTRK1
• NCBI Official Synonym Symbols: MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
• NCBI Protein Information: high affinity nerve growth factor receptor
• UniProt Protein Name: High affinity nerve growth factor receptor
• UniProt Gene Name: NTRK1
• UniProt Synonym Gene Names: MTC; TRK; TRKA; Trk-A
• UniProt Entry Name: NTRK1_HUMAN

NCBI Description

This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq, Jul 2008]

Uniprot Description

TrkA: a receptor tyrosine kinase of the Trk family. High affinity receptor for nerve growth factor, neurotrophin-3 and neurotrophin-4/5 but not brain- derived neurotrophic factor. Known substrates include Shc, PI-3K, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system. Two splice variant isoforms have been described.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; Trk family

Chromosomal Location of Human Ortholog: 1q21-q22

Cellular Component: early endosome; endosome; integral to plasma membrane; late endosome; plasma membrane; protein complex; receptor complex

Molecular Function: nerve growth factor binding; nerve growth factor receptor activity; neurotrophin binding; protein binding; protein homodimerization activity; transmembrane receptor protein tyrosine kinase activity

Biological Process: activation of MAPKK activity; activation of NF-kappaB transcription factor; microtubule-based movement; negative regulation of cell proliferation; negative regulation of neuron apoptosis; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of GTPase activity; positive regulation of programmed cell death; positive regulation of protein amino acid phosphorylation; positive regulation of Ras protein signal transduction; protein amino acid autophosphorylation; protein amino acid phosphorylation; sympathetic nervous system development

Disease: Insensitivity To Pain, Congenital, With Anhidrosis; Thyroid Carcinoma, Familial Medullary