Mouse alpha-Tubulin Antibody | anti-TUBA1 antibody

Anti-alpha-Tubulin Biotin

Cat. Num. AAA1801616

• Gene Names: TUBA1A; LIS3; TUBA3; B-ALPHA-1
• Reactivity: Mouse, Pig, Human, Turkey, Eisenia, Paramecium, Nicotiana, Yeast
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Synonyms: alpha-Tubulin; Anti-alpha-Tubulin Biotin; tubulin alpha 1; TUBA; anti-TUBA1 antibody

• Host: Mouse
• Reactivity: Mouse, Pig, Human, Turkey, Eisenia, Paramecium, Nicotiana, Yeast
• Isotype: IgG1
• Clone Number: TU-01
• Specificity: The antibody TU-01 recognizes a defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.
• Purity/Purification: The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 451

• Applicable Applications for anti-TUBA1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
• Immunogen: Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.
• Conjugation: Biotin
• Preparation and Storage: Store at 2-8 degree C.

Western Blot (WB)

(Western blotting analysis of alpha-tubulin in porcine brain lysate by antibody TU-01 biotin.)

Related Product Information for anti-TUBA1 antibody

The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening - this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression.Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.

NCBI and Uniprot Product Information

• NCBI GI #: 55977864
• NCBI GeneID: 7846
• UniProt Accession #: Q71U36
• NCBI Official Full Name: Tubulin alpha-1A chain
• NCBI Official Synonym Full Names: tubulin alpha 1a
• NCBI Official Symbol: TUBA1A
• NCBI Official Synonym Symbols: LIS3; TUBA3; B-ALPHA-1
• NCBI Protein Information: tubulin alpha-1A chain
• UniProt Protein Name: Tubulin alpha-1A chain
• Protein Family: Alpha-tubulin N-acetyltransferase
• UniProt Gene Name: TUBA1A
• UniProt Synonym Gene Names: TUBA3
• UniProt Entry Name: TBA1A_HUMAN

NCBI Description

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blot studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, intellectual disability, and early-onset epilepsy caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2017]

Uniprot Description

TUBA1A: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Expressed at a high level in fetal brain. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: microtubule; recycling endosome; cytoplasmic microtubule; cytosol; nucleus

Molecular Function: GTPase activity; protein domain specific binding; protein binding; GTP binding; structural constituent of cytoskeleton; structural molecule activity

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; cell division; organelle organization and biogenesis; cytoskeleton-dependent intracellular transport; mitotic cell cycle; G2/M transition of mitotic cell cycle; microtubule-based process

Disease: Lissencephaly 3