Rabbit TOMM20 Monoclonal Antibody | anti-TOMM20 antibody

TOMM20 Rabbit Monoclonal Antibody

Cat. Num. AAA3014141

• Gene Names: TOMM20; MAS20; MOM19; TOM20
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Protein A affinity purified
• Synonyms: TOMM20; Monoclonal Antibody; TOMM20 Rabbit Monoclonal Antibody; Mitochondrial import receptor subunit TOM20 homolog; Mitochondrial 20 kDa outer membrane protein; Outer mitochondrial membrane receptor Tom20; TOM20; KIAA0016; anti-TOMM20 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Specificity: This antibody detects endogenous levels of TOMM20 and does not cross-react with related proteins.
• Purity/Purification: Protein A affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 145

• Applicable Applications for anti-TOMM20 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-1:2000; IHC: 1:50-1:200; ICC: 1:50-1:200; IF: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: Recombinant antibody.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TOMM20 Rabbit mAb at 1:1000 dilutionLane1:Hela whole cell lysateLane2:MCF7 whole cell lysateLane3:F9 whole cell lysateLane4:PC12 whole cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TOMM20 antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining TOMM20 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TOMM20 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TOMM20 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-TOMM20 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-TOMM20 antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining TOMM20 in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Hela cells with TOMM20 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

Related Product Information for anti-TOMM20 antibody

The mitochondrial preprotein translocases of the outer membrane (Tom) is a multisubunit protein complex that facilitates the import of nucleus-encoded precursor proteins across the mitochondrial outer membrane. The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria. The import receptors include Tom20 and Tom22, which form a heteromeric receptor complex that initiates the insertion of newly synthesized proteins into the outer membrane and then directs the precursor protein into the GIP. In yeast, Tom22 is the essential component of the import receptor complex as it functions as both a receptor for the preproteins and serves as a docking point for both Tom20 and the GIP.

NCBI and Uniprot Product Information

• NCBI GI #: 7657257
• NCBI GeneID: 9804
• NCBI Accession #: NP_055580.1
• NCBI GenBank Nucleotide #: NP_055580.1
• UniProt Accession #: Q15388
• Molecular Weight: ~ 16kDa
• NCBI Official Full Name: mitochondrial import receptor subunit TOM20 homolog
• NCBI Official Synonym Full Names: translocase of outer mitochondrial membrane 20
• NCBI Official Symbol: TOMM20
• NCBI Official Synonym Symbols: MAS20; MOM19; TOM20
• NCBI Protein Information: mitochondrial import receptor subunit TOM20 homolog
• UniProt Protein Name: Mitochondrial import receptor subunit TOM20 homolog
• Protein Family: TOMM20-like protein
• UniProt Gene Name: TOMM20
• UniProt Synonym Gene Names: KIAA0016
• UniProt Entry Name: TOM20_HUMAN

Uniprot Description

TOM20: Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. Belongs to the Tom20 family.

Protein type: Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 1q42

Cellular Component: mitochondrion; mitochondrial outer membrane translocase complex; integral to membrane

Molecular Function: protein binding; P-P-bond-hydrolysis-driven protein transmembrane transporter activity; unfolded protein binding

Biological Process: cellular protein metabolic process; protein targeting to mitochondrion