Rat Heme Oxygenase 1, Decycling ELISA Kit | HO1 elisa kit

Rat Heme Oxygenase 1, Decycling ELISA Kit

Cat. Num. AAA727755

• Gene Names: Hmox1; Ho1; Heox; Hmox; Ho-1; HEOXG; hsp32
• Reactivity: Rat
• Synonyms: Heme Oxygenase 1; Decycling; Rat Heme Oxygenase 1; Decycling ELISA Kit; HO1 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/ml
• Intended Uses: This HO1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat HO1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HO1 elisa kit

Principle of the assay: HO1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HO1 antibody and an HO1-HRP conjugate. The assay sample and buffer are incubated together with HO1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HO1 concentration since HO1 from samples and HO1-HRP conjugate compete for the anti-HO1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HO1 from the sample, fewer sites are left to bind HO1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HO1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for HO1 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 6981032
• NCBI GeneID: 24451
• NCBI Accession #: NP_036712.1
• NCBI GenBank Nucleotide #: NM_012580.2
• Molecular Weight: 33,006 Da
• NCBI Official Full Name: heme oxygenase 1
• NCBI Official Synonym Full Names: heme oxygenase (decycling) 1
• NCBI Official Symbol: Hmox1
• NCBI Official Synonym Symbols: Ho1; Heox; Hmox; Ho-1; HEOXG; hsp32
• NCBI Protein Information: heme oxygenase 1; heme oxygenase (decyclizing) 1
• UniProt Protein Name: Heme oxygenase 1
• UniProt Gene Name: Hmox1
• UniProt Synonym Gene Names: HO-1
• UniProt Entry Name: HMOX1_RAT

NCBI Description

catalyzes the oxidative cleavage of heme to biliverdin [RGD, Feb 2006]

Uniprot Description

HMOX1: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 1 activity is highly inducible by its substrate heme and by various non-heme substances such as heavy metals, bromobenzene, endotoxin, oxidizing agents and UVA. Expressed at higher levels in renal cancer tissue than in normal tissue. Belongs to the heme oxygenase family.

Protein type: Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.14.99.3; Oxidoreductase

Cellular Component: endoplasmic reticulum membrane; perinuclear region of cytoplasm; endoplasmic reticulum; nucleolus; plasma membrane; caveola; cytosol; nucleus

Molecular Function: signal transducer activity; protein binding; protein homodimerization activity; enzyme binding; metal ion binding; phospholipase D activity; heme binding; oxidoreductase activity; heme oxygenase (decyclizing) activity

Biological Process: response to nicotine; cell death; negative regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell proliferation; negative regulation of mast cell degranulation; heme metabolic process; negative regulation of cell proliferation; erythrocyte homeostasis; regulation of transcription factor activity; heme catabolic process; regulation of blood pressure; small GTPase mediated signal transduction; phospholipid metabolic process; negative regulation of mast cell cytokine production; negative regulation of neuron apoptosis; angiogenesis; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; healing during inflammatory response; protein homooligomerization; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transcription factor activity; heme oxidation; cellular response to nutrient; negative regulation of DNA binding; iron ion homeostasis; positive regulation of angiogenesis; DNA damage response, signal transduction resulting in induction of apoptosis; response to hydrogen peroxide; response to estrogen stimulus; response to hypoxia; response to oxidative stress