Flow Cytometry (FC/FACS)
(Intracellular Flow Cytometry analysis of TLR8 in 1x10e6 human PBMCs using 0.5ug of MBS606491, 0.25ug of C2265-55B1 anti-human CD14 and 0.5ug of isotype control.)
Mouse anti-Human, Mouse TLR8 Monoclonal Antibody
TLR8 (Toll Like Receptor 8, Toll-like Receptor 8, TLR 8, CD288, CD288 Antigen, MGC119599, MGC119600) (PE)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: Flow Cytometry: 0.5-2ug labels 1x10e6 cells. Ramos cells can be used as a positive control.
Western Blot: A band of ~120kD was detected with transfected cell lysates.
Flow Cytometry: Raw or Raw cells
Flow Cytometry (FC/FACS)
(Intracellular Flow Cytometry analysis of TLR8 in 1x10e6 human PBMCs using 0.5ug of MBS606491, 0.25ug of C2265-55B1 anti-human CD14 and 0.5ug of isotype control.)
Flow Cytometry (FC/FACS)
(Intracellular Flow Cytometry analysis of TLR8 in 1x10e6 human PBMCs using 0.5ug of MBS606491, 0.25ug of C2265-55B1 anti-human CD14 and 0.5ug of isotype control.)
Testing Data
(Intracellular flow analysis of TLR8 in Ramos cells using 2ug of MBS606491. Shaded histogram represents Ramos cells without antibody; green represents isotype control; red represents MBS606491)
Testing Data
(Intracellular flow analysis of TLR8 in Ramos cells using 2ug of MBS606491. Shaded histogram represents Ramos cells without antibody; green represents isotype control; red represents MBS606491)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung and peripheral blood leukocytes, and lies in close proximity to another family member, TLR7, on chromosome X. [provided by RefSeq, Jul 2008]
Uniprot Description
TLR8: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Belongs to the Toll-like receptor family.
Protein type: Receptor, misc.; Membrane protein, integral
Chromosomal Location of Human Ortholog: Xp22
Cellular Component: Golgi membrane; endoplasmic reticulum membrane; integral to membrane; endosome membrane
Molecular Function: single-stranded RNA binding; DNA binding; RNA binding; double-stranded RNA binding; receptor activity; drug binding
Biological Process: I-kappaB kinase/NF-kappaB cascade; regulation of cytokine secretion; response to virus; positive regulation of interleukin-8 biosynthetic process; microglial cell activation; positive regulation of interferon-alpha biosynthetic process; toll-like receptor 8 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interferon-beta biosynthetic process; positive regulation of innate immune response; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; immunoglobulin mediated immune response; inflammatory response; defense response to virus; positive regulation of interferon-gamma biosynthetic process
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Product Notes
The TLR8 (Catalog #AAA606491) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TLR8 (Toll Like Receptor 8, Toll-like Receptor 8, TLR 8, CD288, CD288 Antigen, MGC119599, MGC119600) (PE) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TLR8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry and Western Blot. Dilution: Flow Cytometry: 0.5-2ug labels 1x10e6 cells. Ramos cells can be used as a positive control. Western Blot: A band of ~120kD was detected with transfected cell lysates. Researchers should empirically determine the suitability of the TLR8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TLR8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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