Mouse TLR5 Monoclonal Antibody

TLR5 (Toll Like Receptor 5, Toll-like Receptor 5 Precursor, TLR 5, TLR-5, FLJ10052, MGC126430, MGC126431, Toll/interleukin 1 Receptor-like Protein 3, TIL3)

Cat. Num. AAA603219

• Reactivity: Canine, Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: TLR5; Monoclonal Antibody; TLR5 (Toll Like Receptor 5; Toll-like Receptor 5 Precursor; TLR 5; TLR-5; FLJ10052; MGC126430; MGC126431; Toll/interleukin 1 Receptor-like Protein 3; TIL3); Anti -TLR5 (Toll Like Receptor 5; anti-TLR5 antibody

• Host: Mouse
• Reactivity: Canine, Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 4H281
• Specificity: Recognizes human TLR5. Species Crossreactivity: mouse and canine
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

• Applicable Applications for anti-TLR5 antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Flow Cytometry and Immunohistochemistry.; Dilution: Flow Cytometry: 0.5-1ug per 1x10e6 cells; Western Blot: 1-3ug/ml; 60kD band is detected in transfected cells, corresponding to a truncated form of TLR5.; Immunohistochemistry (Frozen): 10ug/ml; Immunohistochemistry (Paraffin): 5ug/ml
• Immunogen: Synthetic peptide corresponding to a portion of human TLR5 between amino acids 700-800 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of TLR5 in human Ramos cell lysate (A) and mouse Raw cell lysate (B) using MBS603219 at 2ug/ml.)

Testing Data

(Intracellular flow analysis of TLR5 in human monocytes using 0.5 ug of MBS603219. Left histogram: cells without antibody; Middle: isotype control; Right: TLR5 antibody. IgG (PE) Gt x Mo was used as secondary antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of TLR5 in formalin-fixed, paraffin-embedded mouse brain tissue using an isotype control (top) and MBS603219 (bottom) at 5ug/ml.)

Flow Cytometry (FC/FACS)

(Intracellular FACS analysis of TLR5 in 10e6 Ramos cells using (A) 2 ugs of isotype control and (B) 2ugs f MBS603219)

Related Product Information for anti-TLR5 antibody

The Toll-like receptor (TLR) family in mammals comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described (3).

Product Categories/Family for anti-TLR5 antibody

Antibodies; Abs to Toll Like Receptors (TLR)

NCBI and Uniprot Product Information

• NCBI GI #: 237871665
• NCBI Official Full Name: TLR5
• Protein Family: Toll-like receptor