Rabbit anti-Human TLR8 Polyclonal Antibody

TLR8 (Toll Like Receptor 8, Toll-like Receptor 8, TLR 8, CD288, CD288 Antigen, MGC119599, MGC119600)

Cat. Num. AAA622324

• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry
• Purity: Highly Purified; Purified by Ion Exchange chromatography
• Synonyms: TLR8; Polyclonal Antibody; TLR8 (Toll Like Receptor 8; Toll-like Receptor 8; TLR 8; CD288; CD288 Antigen; MGC119599; MGC119600); Anti -TLR8 (Toll Like Receptor 8; anti-TLR8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TLR8. Species Crossreactivity: Mouse
• Purity/Purification: Highly Purified; Purified by Ion Exchange chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide

• Applicable Applications for anti-TLR8 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot and Immunocytochemistry.; Dilution: Western Blot: 0.5-2ug/ml; Immunohistochemistry: 2ug/ml
• Immunogen: Raised against a peptide corresponding to 16 amino acids near the middle of human TLR8 (GenBank accession no. NP_619542)
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of TLR8 in Daudi cell lysates with MBS622324 at (A) 0.5, (B) 1, and (C) 2 ug/ml.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of MBS622324.)

Testing Data

Related Product Information for anti-TLR8 antibody

Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1-3). These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors (4,5). Like TLR7, TLR8 is localized to endosomal or lysosomal compartments (6) and stimulates the innate immune response after activation by guanosine- and uridine-rich single-stranded RNA (7). Human but not murine TLR8 confers responsiveness to the antiviral compound R-848 (8).

Product Categories/Family for anti-TLR8 antibody

Antibodies; Abs to Toll Like Receptors (TLR)

NCBI and Uniprot Product Information

• NCBI GI #: 74054102
• NCBI Official Full Name: TLR8
• Protein Family: Toll-like receptor

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung and peripheral blood leukocytes, and lies in close proximity to another family member, TLR7, on chromosome X. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR8: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: Xp22

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; integral to membrane; endosome membrane

Molecular Function: single-stranded RNA binding; DNA binding; RNA binding; double-stranded RNA binding; receptor activity; drug binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; regulation of cytokine secretion; response to virus; positive regulation of interleukin-8 biosynthetic process; microglial cell activation; positive regulation of interferon-alpha biosynthetic process; toll-like receptor 8 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interferon-beta biosynthetic process; positive regulation of innate immune response; toll-like receptor signaling pathway; innate immune response; immunoglobulin mediated immune response; toll-like receptor 9 signaling pathway; inflammatory response; defense response to virus; positive regulation of interferon-gamma biosynthetic process