Rat anti-Mouse TLR4/MD-2 Complex, TLR4 Monoclonal Antibody | anti-TLR4 antibody

TLR4/MD-2 Complex, TLR4 (Toll Like Receptor 4/MD-2, Toll Like Receptor 4) (PE)

Cat. Num. AAA604266

• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified; Purified
• Synonyms: TLR4/MD-2 Complex; TLR4; Monoclonal Antibody; TLR4 (Toll Like Receptor 4/MD-2; Toll Like Receptor 4) (PE); Anti -TLR4/MD-2 Complex; anti-TLR4 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 4H273
• Specificity: Recognizes mouse TLR4 (Toll Like Receptor 4) (PE)
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-TLR4 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry., Recommended Dilution:, Flow Cytometry: 0.5-2 ug/1 x 10^6 Raw cells., preferentially recognizes TLR4-MD-2 complex than of TLR4 alone., Optimal dilutions to be determined by the researcher.
• Immunogen: Ba/F3 cell line expressing mouse TLR4 and MD-2
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(FACS analysis of cell surface TLR4 using T8050-30Eat 0.625 ug/10^6 Raw cells (pretreated with anti-CD16/32). The shaded histogram represents the isotype control.)

Related Product Information for anti-TLR4 antibody

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 835 amino acids with approximate molecular weight of 90kD (6).

Product Categories/Family for anti-TLR4 antibody

Antibodies; Abs to Toll Like Receptors (TLR)

NCBI and Uniprot Product Information

• NCBI GI #: 153792596
• NCBI GeneID: 100066890
• NCBI Accession #: NP_001093239.1
• NCBI GenBank Nucleotide #: NM_001099769.1
• UniProt Accession #: Q9MYW3
• NCBI Official Full Name: toll-like receptor 4
• NCBI Official Symbol: TLR4
• NCBI Protein Information: toll-like receptor 4
• UniProt Protein Name: Toll-like receptor 4
• Protein Family: Toll-like receptor
• UniProt Entry Name: TLR4_HORSE

Uniprot Description

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- ().