Mouse anti-Human, rat Thyroid Hormone Receptor, a1/a2-Isotype Monoclonal Antibody | anti-THRA antibody

Anti-Thyroid Hormone Receptor, alpha1/alpha2-Isotype

Cat. Num. AAA500019

• Gene Names: THRA; AR7; EAR7; ERBA; CHNG6; ERBA1; NR1A1; THRA1; THRA2; ERB-T-1; c-ERBA-1
• Reactivity: Human, rat
• Applications: Western Blot
• Purity: Protein G purified (Prepared from mouse ascites by ammonium sulfate precipitation followed by affinity purification on a protein G column.)
• Synonyms: Thyroid Hormone Receptor; a1/a2-Isotype; Monoclonal Antibody; Anti-Thyroid Hormone Receptor; alpha1/alpha2-Isotype; anti-THRA antibody

• Host: Mouse
• Reactivity: Human, rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2103
• Specificity: Specific for the ~50k TR-alpha1 and the ~58k TR-alpha2 proteins.
• Purity/Purification: Protein G purified (Prepared from mouse ascites by ammonium sulfate precipitation followed by affinity purification on a protein G column.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 451

• Applicable Applications for anti-THRA antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Peptide corresponding to amino acid residues from the N-terminal region of human thyroid hormone receptor, alpha1/alpha2-isotype.
• Immunogen Information: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH
• Immunogen Species: Human
• Reactivity Assumed Based on 100% Sequence Homology: Canine, mouse, non-human primates
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with human and rat tissue. It is anticipated that the antibody will react with canine, mouse and non-human primates based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Thyroid hormones are essential for development of the central nervous system and deficits in these hormones during development affects such cognitive functions as learning and memory (Ambrogini et al., 2005; Chan and Kilby, 2000). Thyroid hormones exert their physiological role mainly through binding to specific nuclear receptors including the predominant isoforms of thyroid hormone receptors TRalpha1, TRalpha2, TRbeta1 and TRbeta2. TRalpha1, TRbeta1 and TRbeta2 bind T3 with high affinity and also bind to thyroid hormone response elements (TREs) on chromatin to regulate the transcriptional processes in several target tissues, including adult rat brain (Constantinou et al., 2005).
• Preparation and Storage: Store at -20 degree C; stable for at least 1 year.

Testing Data

Related Product Information for anti-THRA antibody

Affinity purified mouse monoclonal antibody

References

• Ambrogini P, Cuppini R, Ferri P, Mancini C, Ciaroni S, Voci A, Gerdoni E, Gallo G (2005) Thyroid hormones affect neurogenesis in the dentate gyrus of adult rat. Neuroendocrinology 81:244-253.
• Chan S, Kilby MD (2000) Thyroid hormone and central nervous system development. J Endocrinol 165:1-8.
• Constantinou C, Margarity M, Valcana T (2005) Region-specific effects of hypothyroidism on the relative expression of thyroid hormone receptors in adult rat brain. Mol Cell Biochem 278:93-100.

NCBI and Uniprot Product Information

• NCBI GI #: 300116307
• NCBI GeneID: 7067
• NCBI Accession #: NP_001177847.1
• NCBI GenBank Nucleotide #: NM_001190918.1
• UniProt Accession #: P10827; P21205; Q8N6A1; Q96H73; A8K3B5
• Molecular Weight: 50/58
• NCBI Official Full Name: thyroid hormone receptor alpha isoform 3
• NCBI Official Synonym Full Names: thyroid hormone receptor, alpha
• NCBI Official Symbol: THRA
• NCBI Official Synonym Symbols: AR7; EAR7; ERBA; CHNG6; ERBA1; NR1A1; THRA1; THRA2; ERB-T-1; c-ERBA-1
• NCBI Protein Information: thyroid hormone receptor alpha; EAR-7; c-erbA-alpha; ERBA-related 7; V-erbA-related protein 7; triiodothyronine receptor; nuclear receptor subfamily 1 group A member 1; thyroid normone nuclear receptor alpha variant 1; thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian)
• UniProt Protein Name: Thyroid hormone receptor alpha
• Protein Family: Thyroid hormone receptor
• UniProt Gene Name: THRA
• UniProt Synonym Gene Names: EAR7; ERBA1; NR1A1; THRA1; THRA2; EAR-7
• UniProt Entry Name: THA_HUMAN

NCBI Description

The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine. Ref.14 Ref.18 Ref.20 Ref.21

Subunit structure: Binds DNA as a dimer; homodimer and heterodimer with RXRB. Interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Probably interacts with SFPQ. Interacts with C1D

By similarity. Interacts with AKAP13. Interacts with TP53INP2. Ref.11 Ref.12 Ref.13 Ref.15 Ref.19

Subcellular location: Nucleus.

Domain: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

Involvement in disease: Hypothyroidism, congenital, non-goitrous, 6 (CHNG6) [MIM:614450]: A disease characterized by growth retardation, developmental retardation, skeletal dysplasia, borderline low thyroxine levels and high triiodothyronine levels. There is differential sensitivity to thyroid hormone action, with retention of hormone responsiveness in the hypothalamic pituitary axis and liver but skeletal, gastrointestinal, and myocardial resistance.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.17

Sequence similarities: Belongs to the nuclear hormone receptor family. NR1 subfamily.Contains 1 nuclear receptor DNA-binding domain.