Rabbit anti-Human, Mouse ERF Polyclonal Antibody | anti-ERF antibody

ERF (E520) Polyclonal Antibody

Cat. Num. AAA3001605

• Gene Names: ERF; PE2; CRS4; PE-2; CHYTS
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ERF; Polyclonal Antibody; ERF (E520) Polyclonal Antibody; ETS domain containing transcription factor ERF; ETS domain-containing transcription factor ERF; Ets2 repressor factor; PE 2; PE-2; PE2; anti-ERF antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ERF (E520) polyclonal antibody detects endogenous levels of ERF protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 548

• Applicable Applications for anti-ERF antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 500-550 of Human ERF.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ERF (E520) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-ERF antibody

ABT1 (activator of basal transcription 1) is a nuclear protein that associates with the TATA-binding protein (TBP) and enhances basal transcription activity of class II promoters. ABT1 associates with TBP in HeLa nuclear extracts in vitro. Another protein, designated ERF, is a member of the Ets family of transcription factors. The members of the Ets family are grouped because they share a highly conserved DNA binding domain. These factors are involved in growth factor pathways and regulate both proliferation and differentiation. ERF (Ets-2 repressor factor) is a ubiquitously expressed Ets-domain protein that exhibits strong transcriptional repressor activity, suppresses Ets-induced transformation and is regulated by MAPK phosphorylation. ERF transcription may be regulated by Ets-domain proteins. Additionally, modulation of ERF activity is involved in the transcriptional regulation of genes activated during entry into G1 phase.

NCBI and Uniprot Product Information

• NCBI GI #: 156104872
• NCBI GeneID: 2077
• NCBI Accession #: NP_006485.2
• NCBI GenBank Nucleotide #: NP_006485.2
• UniProt Accession #: P50548
• Molecular Weight: 60, 90
• NCBI Official Full Name: ETS domain-containing transcription factor ERF isoform 1
• NCBI Official Synonym Full Names: ETS2 repressor factor
• NCBI Official Symbol: ERF
• NCBI Official Synonym Symbols: PE2; CRS4; PE-2; CHYTS
• NCBI Protein Information: ETS domain-containing transcription factor ERF
• UniProt Protein Name: ETS domain-containing transcription factor ERF
• Protein Family: eRF1 methyltransferase
• UniProt Gene Name: ERF
• UniProt Entry Name: ERF_HUMAN

NCBI Description

ETS2 is a transcription factor and protooncogene involved in development, apoptosis, and the regulation of telomerase. The protein encoded by this gene binds to the ETS2 promoter and is a strong repressor of ETS2 transcription. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]

Uniprot Description

ERF: potent transcriptional repressor that binds to the H1 element of the ETS2 promoter. Belongs to the ETS family of transcription factors. Its function is regulated by phosphorylation.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: nucleoplasm; cytosol; nucleus

Molecular Function: sequence-specific DNA binding; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; cell cycle; cell differentiation

Disease: Craniosynostosis 4