Typical Testing Data/Standard Curve (for reference only)
Human IL-1 R alpha ELISA Kit | IL-1 R alpha elisa kit
IL-1 R alpha Human ELISA Kit
Reactivity
Human
Applications
ELISA
Synonyms
IL-1 R alpha; IL-1 R alpha Human ELISA Kit; Human IL-1 R alpha; IL-1 R alpha elisa kit
Reactivity
Human
Applicable Applications for IL-1 R alpha elisa kit
Quantitative sELISA (EIA)
Application Notes
For quantitative detection of IL-1RA in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Assay Type
Sandwich
Detection Range
31.2 pg/ml-2000 pg/ml
Sensitivity
< 2pg/ml
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL-1 R alpha elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-1RA polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IL-1RA polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-1RA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-1RA can be calculated.
Background: The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene which maps to 2q14.2. It is a member of the interleukin 1 cytokine family, and it is secreted by various types of cells including immune cells, epithelial cells, and adipocytes, IL-1RA is an agent that binds non-productively to the cell surface interleukin-1 receptor (IL-1R). Carter et al. (1990) found that IL1RN specifically inhibited IL1 bioactivity on T cells and endothelial cells in vitro and was a potent inhibitor of IL1-induced corticosterone production in vivo. Mutations in the IL1RN gene results in a rare disease called deficiency of the interleukin-1-receptor antagonist (DIRA), and elevated levels of IL1RN has been found in serum of schizophrenia patients.
Background: The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene which maps to 2q14.2. It is a member of the interleukin 1 cytokine family, and it is secreted by various types of cells including immune cells, epithelial cells, and adipocytes, IL-1RA is an agent that binds non-productively to the cell surface interleukin-1 receptor (IL-1R). Carter et al. (1990) found that IL1RN specifically inhibited IL1 bioactivity on T cells and endothelial cells in vitro and was a potent inhibitor of IL1-induced corticosterone production in vivo. Mutations in the IL1RN gene results in a rare disease called deficiency of the interleukin-1-receptor antagonist (DIRA), and elevated levels of IL1RN has been found in serum of schizophrenia patients.
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Product Notes
The Human IL-1 R alpha (Catalog #AAA355280) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA355280 ELISA Kit recognizes Human IL-1 R alpha. AAA Biotech's IL-1 R alpha can be used in a range of immunoassay formats including, but not limited to, Quantitative sELISA (EIA). For quantitative detection of IL-1RA in human serum, plasma, body fluids, tissue lysates or cell culture supernatants. Researchers should empirically determine the suitability of the IL-1 R alpha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-1 R alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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