Mouse Npc1 (NPC intracellular cholesterol transporter 1) ELISA Kit | Npc1 elisa kit

Mouse Npc1 (NPC intracellular cholesterol transporter 1) ELISA Kit

Cat. Num. AAA7612396

• Gene Names: Npc1; spm; lcsd; C85354; nmf164; D18Ertd139e; D18Ertd723e; A430089E03Rik
• Reactivity: Mouse
• Synonyms: Npc1 (NPC intracellular cholesterol transporter 1); Mouse Npc1 (NPC intracellular cholesterol transporter 1) ELISA Kit; NPC intracellular cholesterol transporter 1; Niemann-Pick C1 protein; Npc1; Npc1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Npc1. No significant cross-reactivity or interference between Npc1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Npc1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 89242146
• NCBI GeneID: 18145
• NCBI Accession #: NP_032746.2
• NCBI GenBank Nucleotide #: NM_008720.2
• UniProt Accession #: O35604; O35605; G3X8W9
• Molecular Weight: 142,885 Da
• NCBI Official Full Name: Niemann-Pick C1 protein
• NCBI Official Synonym Full Names: Niemann-Pick type C1
• NCBI Official Symbol: Npc1
• NCBI Official Synonym Symbols: spm; lcsd; C85354; nmf164; D18Ertd139e; D18Ertd723e; A430089E03Rik
• NCBI Protein Information: Niemann-Pick C1 protein
• UniProt Protein Name: Niemann-Pick C1 protein
• Protein Family: Niemann-Pick C1 protein
• UniProt Gene Name: Npc1
• UniProt Entry Name: NPC1_MOUSE

Uniprot Description

NPC1: Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals. Defects in NPC1 are the cause of Niemann-Pick disease type C1 (NPC1). A lysosomal storage disorder that affects the viscera and the central nervous system. It is due to defective intracellular processing and transport of low-density lipoprotein derived cholesterol. It causes accumulation of cholesterol in lysosomes, with delayed induction of cholesterol homeostatic reactions. Niemann-Pick disease type C1 has a highly variable clinical phenotype. Clinical features include variable hepatosplenomegaly and severe progressive neurological dysfunction such as ataxia, dystonia and dementia. The age of onset can vary from infancy to late adulthood. An allelic variant of Niemann-Pick disease type C1 is found in people with Nova Scotia ancestry. Patients with the Nova Scotian clinical variant are less severely affected. Belongs to the patched family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: Golgi apparatus; lysosomal membrane; endoplasmic reticulum; lysosome; integral to plasma membrane; integral to membrane; extracellular region; nuclear envelope; lipid raft; membrane; perinuclear region of cytoplasm; plasma membrane; endosome; vesicle

Molecular Function: protein binding; hedgehog receptor activity; cholesterol binding

Biological Process: response to drug; steroid metabolic process; cholesterol metabolic process; cholesterol transport; protein amino acid glycosylation; endocytosis; cholesterol efflux; adult walking behavior; negative regulation of macroautophagy; cholesterol homeostasis; response to cadmium ion; autophagy; lipid metabolic process; lipid raft organization and biogenesis