Mouse Thymidylate Synthase Monoclonal Antibody | anti-TYMS antibody

Anti-Thymidylate Synthase Monoclonal Antibody

Cat. Num. AAA190052

• Gene Names: TYMS; TS; TMS; HST422; MGC88736
• Applications: ELISA, Immunoblot, Western Blot, Immunohistochemistry, Immunoprecipitation
• Synonyms: Thymidylate Synthase; Monoclonal Antibody; Anti-Thymidylate Synthase Monoclonal Antibody; Anti-Thymidylate Synthase; anti-TYMS antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Anti-Thymidylate Synthase [Murine MAb to human thymidylate synthase, a drug resistance-associated enzyme.]; These antibodies specifically recognize human TS (36 kDa). They do not crossreact with rat TS.
• Form/Format: 200 ug Protein G-purified antibody in PBS, pH 7.4.
• Sequence Length: 313

• Applicable Applications for anti-TYMS antibody: ELISA, Immunoblot, Immunohistochemistry, Immunoprecipitation
• Application Notes: Immunohistochemistry: use at 5-10 ug/ml on frozen sections (reactivity on paraffin-embedded sections has not been determined).; Immunoblotting: use at 5-10 ug/ml. ELISA: use at 2-5 ug/ml (optimized for TS on solid phase at 5 ug/ml).; Immunoprecipitation: use #34002 only at 5-10 ug/ml.
• Antigen: Recombinant human thymidylate synthase produced in E. coli.
• Stabilizer: None
• Preservatives: None. Available on request.
• Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

Related Product Information for anti-TYMS antibody

Thymidylate synthase (TS) is an intracellular enzyme critical for de novo synthesis of DNA. In cancer, expression of TS is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of TS activity. Overexpression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma and is usually predictive of shortened survival time.

Product Categories/Family for anti-TYMS antibody

Cancer Research Products

NCBI and Uniprot Product Information

• NCBI GI #: 4507751
• NCBI GeneID: 7298
• NCBI Accession #: NP_001062.1
• NCBI GenBank Nucleotide #: NM_001071.2
• UniProt Accession #: P04818; Q53FB7; Q53Y97; Q8WYK3; Q8WYK4
• Molecular Weight: 35,716 Da
• NCBI Official Full Name: thymidylate synthase
• NCBI Official Synonym Full Names: thymidylate synthetase
• NCBI Official Symbol: TYMS
• NCBI Official Synonym Symbols: TS; TMS; HST422; MGC88736
• NCBI Protein Information: thymidylate synthase; TSase; OTTHUMP00000162195
• UniProt Protein Name: Thymidylate synthase
• Protein Family: Thymidylate synthase
• UniProt Gene Name: TYMS
• UniProt Synonym Gene Names: TS
• UniProt Entry Name: TYSY_HUMAN

NCBI Description

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq]

Uniprot Description

TYMS: an enzyme in the thymidylate synthase family. Polymorphisms serve as prognostic markers for determining modality of treatment in certain cancers. Genotyping helps predict toxicity to 5-FU-based chemotherapy in patients with colorectal cancer and the response to platinum-based chemotherapy in advanced NSCLC. A common polymorphism is a determinant of red blood cell folate and homocysteine concentrations. Homodimer.

Protein type: DNA repair, damage; Cofactor and Vitamin Metabolism - one carbon pool by folate; Nucleotide Metabolism - pyrimidine; Mitochondrial; EC 2.1.1.45; Methyltransferase

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; mitochondrial inner membrane; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: mRNA binding; protein homodimerization activity; nucleotide binding; drug binding; cofactor binding; thymidylate synthase activity; folic acid binding

Biological Process: immortalization of host cell by virus; uracil metabolic process; circadian rhythm; methylation; tetrahydrofolate metabolic process; developmental growth; response to organophosphorus; response to toxin; response to glucocorticoid stimulus; dTTP biosynthetic process; response to vitamin A; pyrimidine base metabolic process; response to folic acid; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; aging; response to drug; organ regeneration; nucleobase, nucleoside and nucleotide metabolic process; dTMP biosynthetic process; deoxyribonucleoside monophosphate biosynthetic process; G1/S-specific transcription in mitotic cell cycle; response to ethanol; cartilage development; response to cytokine stimulus; pyrimidine nucleoside biosynthetic process; dUMP metabolic process; mitotic cell cycle; response to progesterone stimulus; G1/S transition of mitotic cell cycle