Mouse Threonine Monoclonal Antibody

Threonine, Phosphorylated (Biotin)

Cat. Num. AAA632418

• Applications: ELISA, Western Blot
• Purity: Purified; Purified
• Synonyms: Threonine; Monoclonal Antibody; Phosphorylated (Biotin); Anti -Threonine; anti-Threonine antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 11C156
• Specificity: Recognizes phosphorylated threonine both as free amino acid or when conjugated to carriers such as BSA or KLH. Does not react with nonphosphorylated threonine, phosphorylated tyrosine or serine, AMP, or ATP.
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a lyophilized powder from PBS, 0.2% BSA, 0.05% sodium azide. Labeled with Biotin. Reconstitute with 100ul sterile PBS.

• Applicable Applications for anti-Threonine antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1000-1:5000; Western Blot: 1:500-1:2000
• Immunogen: o-Phospho-L-threonine, conjugated to KLH
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Threonine antibody

Protein phosphorylation and dephosphorylation reactions is a key postranslational event in the modification of protein functions. Protein phosphorylation occurs at tyrosine, serine, or threonine (p-tyr, p-ser, or p-thr) residues. Many different mitogenic systems, such as the EGF, PDGF, and insulin receptor systems contain tyr/ser/thr kinase domains which autophosphorylate specific tyr, ser or thr residues upon binding of their ligands. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated proteins. An understanding of transformation by oncogenes and mitogenic processes of growth factors requires a delineation of all potential partners involved in phosphorylation cascade. The availability of antibodies specific for p-tyr, p-thr or p-thr residues has greatly advanced the studies on role of phosphorylated proteins.

Product Categories/Family for anti-Threonine antibody

Antibodies; Abs to Amino Acids

NCBI and Uniprot Product Information

• NCBI GI #: 386420320
• NCBI Official Full Name: threonine dehydratase, biosynthetic
• Protein Family: Threonine synthase

Uniprot Description

Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA.