Western Blot (WB)
(Western blot analysis of CD222 expression in Jurkay (A), K562 (B), Caco2 (C) whole cell lysates.)
Rabbit CD222 Polyclonal Antibody | anti-IGF2R antibody
Anti-CD222 Antibody
Western Blot (WB)
(Western blot analysis of CD222 expression in Jurkay (A), K562 (B), Caco2 (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of CD222 expression in Jurkay (A), K562 (B), Caco2 (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of CD222 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of CD222 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF)
(Immunofluorescent analysis of CD222 staining in Jurkay cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of CD222 staining in Jurkay cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate. The binding sites for each ligand are located on different segments of the protein. This receptor has various functions, including in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. Mutation or loss of heterozygosity of this gene has been association with risk of hepatocellular carcinoma. The orthologous mouse gene is imprinted and shows exclusive expression from the maternal allele; however, imprinting of the human gene may be polymorphic, as only a minority of individuals showed biased expression from the maternal allele (PMID:8267611). [provided by RefSeq, Nov 2015]
Uniprot Description
IGF2R: a multifunctional type I transmembrane protein receptor that binds insulin-like growth factor 2 at the cell surface and mannose-6-phosphate-tagged proteins in the trans-Golgi network. Also known as the cation-independent mannose-6 phosphate (M6P) receptor. Clears IGF2 from the cell surface to attenuate signaling, and transports M6P-tagged lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. M6P-tagged lysosomal enzymes bind specifically to mannose-6- phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Belongs to the MRL1/IGF2R family.
Protein type: Vesicle; Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 6q26
Cellular Component: cell surface; clathrin coat; early endosome; endocytic vesicle; endosome; extracellular space; focal adhesion; integral to plasma membrane; late endosome; lysosomal membrane; membrane; nuclear envelope lumen; perinuclear region of cytoplasm; trans-Golgi network; trans-Golgi network transport vesicle
Molecular Function: enzyme binding; G-protein alpha-subunit binding; G-protein coupled receptor activity; glycoprotein binding; identical protein binding; insulin-like growth factor II binding; insulin-like growth factor receptor activity; mannose binding; phosphoprotein binding; protein binding; receptor activity; retinoic acid binding; transporter activity
Biological Process: G-protein coupled receptor protein signaling pathway; insulin-like growth factor receptor signaling pathway; liver development; organ regeneration; positive regulation of apoptosis; post-embryonic development; receptor-mediated endocytosis; response to retinoic acid; signal transduction; spermatogenesis
Disease: Hepatocellular Carcinoma
Research Articles on IGF2R
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Product Notes
The IGF2R igf2r (Catalog #AAA8242816) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD222 Antibody reacts with Human, Mouse, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's CD222 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500). Researchers should empirically determine the suitability of the IGF2R igf2r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD222, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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